Department of Environmental Physiology, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
Sankyo Holdings Co., Ltd, Fuji, Shizuoka 417-0061, Japan.
Food Funct. 2021 May 11;12(9):3992-4004. doi: 10.1039/d0fo03272g.
Learning and memory impairment may result from age-related decline in synaptic plasticity-related proteins in the hippocampus. Therefore, exploration of functional foods capable of ameliorating memory and cognition decline is an interesting endeavor in neuroscience research. We report the effects of Anredera cordifolia (AC) extract on learning and memory deficits in a senescence-accelerated mouse-prone 8 (SAMP8) mouse model, which demonstrate age-related memory deficits and related pathological changes in the brain. After 8 weeks of oral administration of AC extract, the mice were trained in the Novel Object Recognition (NOR) task, and after 7 more weeks, in the Morris Water Maze (MWM) task. Following the completion of behavioral testing, the blood biochemistry parameters, the hippocampal levels of brain-derived neurotropic factor (BDNF), PSD95, and NR2A, and the p-cAMP-response element binding (p-CREB)/CREB ratio were measured. The AC-treated group spent more time exploring the novel objects in the NOR task, and showed faster acquisition and better retention in the MWM task than the negative control (CN) group. In addition, AC enhanced the levels of the aforementioned neuronal plasticity-related proteins, and did not affect the blood biochemistry parameters. Therefore, our data suggest that the AC extract may improve learning and memory without causing any noticeable side effects in the body.
学习和记忆障碍可能是由于海马突触可塑性相关蛋白随年龄增长而下降引起的。因此，探索能够改善记忆和认知能力下降的功能性食品是神经科学研究中的一个有趣领域。我们报告了 anredera cordifolia (AC) 提取物对快速老化小鼠品系 8 (SAMP8) 小鼠模型学习和记忆缺陷的影响，该模型表现出与年龄相关的记忆缺陷和大脑相关的病理变化。在口服 AC 提取物 8 周后，对小鼠进行新物体识别 (NOR) 任务训练，7 周后进行 Morris 水迷宫 (MWM) 任务训练。完成行为测试后，测量血液生化参数、海马脑源性神经营养因子 (BDNF)、PSD95 和 NR2A 水平以及 p-cAMP 反应元件结合 (p-CREB)/CREB 比值。与阴性对照组 (CN) 相比，AC 处理组在 NOR 任务中花费更多时间探索新物体，在 MWM 任务中表现出更快的获取和更好的保留。此外，AC 增强了上述与神经元可塑性相关的蛋白质水平，且对血液生化参数没有影响。因此，我们的数据表明，AC 提取物可能改善学习和记忆，而不会对身体造成任何明显的副作用。
