Leite Alberto Andrade, Reiter Russel Joseph, Brandão Julio Cezar Mendes, Sakae Thiago Mamoru, Marinho Marcia, Camargo Celia Regina, Oliveira-Junior Itamar Souza
Programa de Pos-Graduacao em Medicina Translacional, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR.
Department of Cell Systems and Anatomy, UT Health Science Center at San Antonio, San Antonio, Texas, USA.
Clinics (Sao Paulo). 2021 May 5;76:e2513. doi: 10.6061/clinics/2021/e2513. eCollection 2021.
The current study compared the impact of pretreatment with melatonin and N-acetylcysteine (NAC) on the prevention of rat lung damage following intestinal ischemia-reperfusion (iIR).
Twenty-eight Wistar rats were subjected to intestinal ischemia induced by a 60 min occlusion of the superior mesenteric artery, followed by reperfusion for 120 min. Animals were divided into the following groups (n=7 per group): sham, only abdominal incision; SS+iIR, pretreated with saline solution and iIR; NAC+iIR, pretreated with NAC (20 mg/kg) and iIR; MEL+iIR, pretreated with melatonin (20 mg/kg) and iIR. Oxidative stress and inflammatory mediators were measured and histological analyses were performed in the lung tissues.
Data showed a reduction in malondialdehyde (MDA), myeloperoxidase (MPO), and TNF-alpha in the animals pretreated with NAC or MEL when compared to those treated with SS+iIR (p<0.05). An increase in superoxide dismutase (SOD) levels in the NAC- and MEL-pretreated animals as compared to the SS+iIR group (34±8 U/g of tissue; p<0.05) was also observed. TNF-α levels were lower in the MEL+iIR group (91±5 pg/mL) than in the NAC+iIR group (101±6 pg/mL). Histological analysis demonstrated a higher lung lesion score in the SS+iIR group than in the pretreated groups.
Both agents individually provided tissue protective effect against intestinal IR-induced lung injury, but melatonin was more effective in ameliorating the parameters analyzed in this study.
本研究比较了褪黑素和N-乙酰半胱氨酸（NAC）预处理对预防大鼠肠缺血再灌注（iIR）后肺损伤的影响。
28只Wistar大鼠接受肠系膜上动脉60分钟闭塞诱导的肠缺血，随后再灌注120分钟。动物分为以下几组（每组n = 7）：假手术组，仅做腹部切口；SS + iIR组，用生理盐水预处理后进行iIR；NAC + iIR组，用NAC（20mg/kg）预处理后进行iIR；MEL + iIR组，用褪黑素（20mg/kg）预处理后进行iIR。检测肺组织中的氧化应激和炎症介质，并进行组织学分析。
数据显示，与SS + iIR组相比，NAC或MEL预处理的动物丙二醛（MDA）、髓过氧化物酶（MPO）和肿瘤坏死因子-α（TNF-α）减少（p < 0.05）。与SS + iIR组相比，NAC和MEL预处理的动物超氧化物歧化酶（SOD）水平也有所增加（34±8 U/g组织；p < 0.05）。MEL + iIR组的TNF-α水平（91±5 pg/mL）低于NAC + iIR组（101±6 pg/mL）。组织学分析表明，SS + iIR组的肺损伤评分高于预处理组。
两种药物单独使用均对肠缺血再灌注诱导的肺损伤具有组织保护作用，但褪黑素在改善本研究分析的参数方面更有效。
