Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Am J Clin Pathol. 2021 Oct 13;156(5):717-721. doi: 10.1093/ajcp/aqab022.
We aimed to quantify the measurement uncertainty (MU) for factor activities using the Monte Carlo simulation (MCS), which is a computational algorithm that simulates statistical sampling to obtain numerical results through complex mathematical calculations.
The uncertainties of factor V (FV), factor VIII (FVIII), and factor X (FX) were simulated with two coagulation testing systems: ACL TOP 750 CTS (Instrumentation Laboratory) and STA Compact (Diagnostica Stago).
When the factor activities were 74% (FV), 68% (FVIII), and 89% (FX), the MUs were 3.5% (FV), 9.3% (VIII), and 2.8% (FX) for ACL TOP and 8.5% (FV), 18.2% (FVII), and 6.5% (FX) for STA Compact. MCS MUs were compared with MUs obtained from running actual samples (not simulated) using a method called the top-down approach. The MCS MU results were interchangeable with MUs from the top-down approach, except for FVIII from STA Compact.
The MCS procedure is well suited for the quantification of MUs for factor assays over the entire measurement range.
我们旨在使用蒙特卡罗模拟（MCS）量化因子活性的测量不确定度（MU），这是一种通过复杂的数学计算模拟统计抽样以获得数值结果的计算算法。
使用两种凝血检测系统：ACL TOP 750 CTS（Instrumentation Laboratory）和 STA Compact（Diagnostica Stago）模拟因子 V（FV）、因子 VIII（FVIII）和因子 X（FX）的不确定性。
当因子活性分别为 74%（FV）、68%（FVIII）和 89%（FX）时，ACL TOP 的 MU 分别为 3.5%（FV）、9.3%（VIII）和 2.8%（FX），STA Compact 的 MU 分别为 8.5%（FV）、18.2%（FVII）和 6.5%（FX）。使用称为自上而下方法的方法将 MCS MU 与使用实际样本（未模拟）运行获得的 MU 进行比较。除 STA Compact 的 FVIII 外，MCS MU 结果与自上而下方法的 MU 可互换。
MCS 程序非常适合在整个测量范围内量化因子测定的 MU。
