Furuya S, Kumamoto Y, Suzuki T, Takauji M, Nagai T
Andrologia. 1977 Oct-Dec;9(4):349-56. doi: 10.1111/j.1439-0272.1977.tb01687.x.
Two types of the cytoplasmic filaments are found in human peritubular cells; thin filaments (50-80 A) and 100 A filaments. The thin filaments are numerous and tightly packed in cytoplasma of peripheral cell processes, and form bundles. These bundles are arranged in parallel with the long axis of the cells. Dense areas are occasionally seen among the filaments, or underneath the cell membrane to which the thin filaments seem to be anchored. The 100 A filaments are generally located around the perinuclear region or in the middle region of cytoplasma. The thin filaments are not altered after incubation with Hasselbach-Schneider's solution. These filaments, however, disappear in cytoplasma when extracted with 0.6 M KI solution. And also, the thin filaments form chracteristic arrowhead complexes, when incubated with HMM, while these complexes are never found, when incubated with HMM plus ATP. The 100 A filaments do not react to HMM. Thus it is suggested that the thin filaments are actin or actin like and that a contractile system exists in human peritubular cells, which may be involved in transport of nonmotile sperm or permeability of the seminiferous walls.
在人类的肾小管周细胞中发现了两种类型的细胞质细丝；细肌丝（50 - 80埃）和100埃的细丝。细肌丝数量众多，紧密排列在周边细胞突起的细胞质中，并形成束状。这些束状结构与细胞的长轴平行排列。在细丝之间或细胞膜下方偶尔可见致密区域，细肌丝似乎锚定在该细胞膜上。100埃的细丝通常位于核周区域周围或细胞质的中间区域。用哈塞尔巴赫 - 施奈德溶液孵育后，细肌丝没有变化。然而，当用0.6M KI溶液提取时，这些细丝在细胞质中消失。此外，当与重酶解肌球蛋白（HMM）孵育时，细肌丝形成特征性的箭头状复合物，而当与HMM加ATP孵育时，从未发现这些复合物。100埃的细丝对HMM无反应。因此，提示细肌丝是肌动蛋白或类似肌动蛋白，并且在人类肾小管周细胞中存在一个收缩系统，其可能参与非游动精子的运输或生精管壁的通透性。
