环状 RNA circFADS2 在脓毒症中表达上调，并通过抑制 miR-15a-5p 的成熟来抑制 LPS 诱导的肺细胞凋亡。
Circular RNA circFADS2 is overexpressed in sepsis and suppresses LPS-induced lung cell apoptosis by inhibiting the maturation of miR-15a-5p.
机构信息
Pediatric Intensive Care Unit, The Seventh Medical Center, PLA General Hospital, No. 5 Nanmencang, Dongshitiao, Dongcheng District, Beijing, 100700, P. R. China.
Department of Cardiovascular Surgery, PLA General Hospital, Beijing, 100853, P. R. China.
出版信息
BMC Immunol. 2021 May 12;22(1):29. doi: 10.1186/s12865-021-00419-7.
BACKGROUND
Circular RNA circFADS2 plays protective roles in LPS-induced inflammation, which promotes sepsis, suggesting its involvement in sepsis.
METHODS
Expression of circFADS2, mature miR-15a-5p, and miR-15a-5p precursor in plasma samples from sepsis patients and healthy controls was determined by RT-qPCR. The circFADS2 expression vector was transfected in lung cells, followed by the measurement of the expression levels of mature miR-15a-5p and miR-15a-5p precursor to study the role of circFADS2 in miR-15a-5p maturation. Cell apoptosis was analyzed by cell apoptosis assay.
RESULTS
CircFADS2 was upregulated in sepsis and inversely correlated with mature miR-15a-5p, but not miR-15a-5p precursor. In lung cells, circFADS2 overexpression decreased the level of mature miR-15a-5p, but not miR-15a-5p precursor. LPS treatment decreased miR-15a-5p expression and increased circFADS2 level. Cell apoptosis analysis showed that circFADS2 overexpression reduced miR-15a-5p overexpression-induced apoptosis of LPS-treated lung cells.
CONCLUSIONS
CircFADS2 is upregulated in sepsis to suppress LPS-induced lung cell apoptosis by inhibiting miR-15a-5p maturation.
背景
环状 RNA circFADS2 在 LPS 诱导的炎症中发挥保护作用，促进脓毒症的发生，表明其参与了脓毒症的发生。
方法
通过 RT-qPCR 测定脓毒症患者和健康对照者血浆样本中 circFADS2、成熟 miR-15a-5p 和 miR-15a-5p 前体的表达。转染肺细胞 circFADS2 表达载体，测定成熟 miR-15a-5p 和 miR-15a-5p 前体的表达水平，研究 circFADS2 在 miR-15a-5p 成熟中的作用。通过细胞凋亡分析检测细胞凋亡。
结果
circFADS2 在脓毒症中上调，与成熟 miR-15a-5p 呈负相关，但与 miR-15a-5p 前体无关。在肺细胞中，circFADS2 过表达降低了成熟 miR-15a-5p 的水平，但不降低 miR-15a-5p 前体的水平。LPS 处理降低了 miR-15a-5p 的表达并增加了 circFADS2 的水平。细胞凋亡分析表明，circFADS2 过表达减少了 LPS 处理的肺细胞中 miR-15a-5p 过表达诱导的细胞凋亡。
结论
circFADS2 在脓毒症中上调，通过抑制 miR-15a-5p 的成熟来抑制 LPS 诱导的肺细胞凋亡。
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