Wang Caiyun, Jin Cuiyuan, Tu Wenqing, Jin Yuanxiang
Department of Biotechnology, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Research Institute of Poyang Lake, Jiangxi Academy of Sciences, Nanchang 330029, China.
Endocr J. 2021 Oct 28;68(10):1165-1177. doi: 10.1507/endocrj.EJ20-0781. Epub 2021 May 11.
The toxicity of certain novel perfluoroalkyl substances (PFCs) has attracted increasing attention. However, the toxic effects of sodium p-perfluorous nonenoxybenzene sulfonate (OBS) on the endocrine system have not been elucidated. In this study, OBS was added to the drinking water during the pregnancy and lactation of the healthy female mice at dietary levels of 0.0 mg/L (CON), 0.5 mg/L (OBS-L), and 5.0 mg/L (OBS-H). OBS exposure during the pregnancy and lactation resulted in the presence of OBS residues in the placenta and fetus. We also analyzed physiological and biochemical parameters and gene expression levels in mice of the F0 and F1 generations after maternal OBS exposure. The total serum cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels were significantly increased in female mice of the F0 generation. The androgen levels in the serum and the ovarian mRNA levels of androgen receptor (AR) also tended to increase after maternal OBS exposure in the F0 generation mice. Moreover, maternal OBS exposure altered the mRNA expression of endocrine-related genes in male mice of F1 generation. Notably, the serum TC and LDL-C levels were significantly increased in 8-weeks-old male mice of the F1 generation, and the serum high-density lipoprotein cholesterol (HDL-C) levels were decreased in 24-week-old male mice of the F1 generation. These results indicated that maternal OBS exposure can interfere with endocrine homeostasis in the F0 and F1 generations. Therefore, exposure to OBS during pregnancy and lactation has the potential toxic effects on the dams and male offspring, which cannot be overlooked.
某些新型全氟烷基物质（PFCs）的毒性已引起越来越多的关注。然而，对全氟壬烯氧基苯磺酸钠（OBS）对内分泌系统的毒性作用尚未阐明。在本研究中，在健康雌性小鼠怀孕和哺乳期间，将OBS以0.0 mg/L（CON）、0.5 mg/L（OBS-L）和5.0 mg/L（OBS-H）的膳食水平添加到饮用水中。怀孕和哺乳期间接触OBS导致胎盘和胎儿中存在OBS残留。我们还分析了母体OBS暴露后F0和F1代小鼠的生理生化参数和基因表达水平。F0代雌性小鼠的血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）水平显著升高。F0代小鼠母体OBS暴露后，血清雄激素水平和雄激素受体（AR）的卵巢mRNA水平也有升高趋势。此外，母体OBS暴露改变了F1代雄性小鼠内分泌相关基因的mRNA表达。值得注意的是，F1代8周龄雄性小鼠的血清TC和LDL-C水平显著升高，F1代24周龄雄性小鼠的血清高密度脂蛋白胆固醇（HDL-C）水平降低。这些结果表明，母体OBS暴露可干扰F0和F1代的内分泌稳态。因此，怀孕和哺乳期间接触OBS对母鼠和雄性后代具有潜在毒性作用，不容忽视。
