生成用于 BMI1+ 癌症干细胞谱系示踪的鳞状细胞癌小鼠模型。

Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells.

机构信息

Center for Translational Medicine, Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510030, China.

Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.

出版信息

STAR Protoc. 2021 Apr 18;2(2):100484. doi: 10.1016/j.xpro.2021.100484. eCollection 2021 Jun 18.

DOI:10.1016/j.xpro.2021.100484

PMID:33982017

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8081985/

Abstract

BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the Cre-loxP recombination strategy, which allows us to study the regulation and targeting of BMI1 CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020).

摘要

BMI1 表达的癌症干细胞 (CSCs) 在头颈部鳞状细胞癌 (HNSCC) 的发生、发展、治疗耐药、复发和转移中起关键作用。在这里，我们提供了一个化学诱导的 HNSCC 小鼠模型，在遗传和病理上与人 HNSCC 相似。本方案描述了如何使用基于 Cre-loxP 重组策略的遗传谱系追踪，这使我们能够研究 BMI1 CSCs 在原发性肿瘤和淋巴结转移中的调控和靶向。有关该方案使用和执行的完整详细信息，请参见 Chen 等人 (2017) 和 Jia 等人 (2020)。