Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Embrapa Suínos e Aves, Concordia, Brasil.
Food Sci Technol Int. 2022 Apr;28(4):366-376. doi: 10.1177/10820132211014985. Epub 2021 May 13.
Ozone application has been suggested as an additional measure to the slaughter animals under hygiene programs. In this study, we determined the efficacy of gaseous ozone applied to pig carcasses during chilling (16 h at 2-5°C). Forty carcasses were allocated to each treatment: control, without ozone application (T1) and 5 ppm gaseous ozone application (T2), divided in two 4-h periods. The carcasses were sampled before and after chilling. The average counts of total aerobic mesophilic (TAM) bacteria before chilling were not different (p = 0.55) between T1 and T2. In turn, after chilling, the ozone-treated carcasses had significantly reduced about 0.4 colony-forming units (CFU)/cm of TAM counts (p < 0.001) than the control carcasses. No significant reduction was observed in the number of carcasses positive for sp. and after gaseous ozone treatment; while a tendency (p = 0.08) of lower number of positive carcasses in T2 was observed. Common macrorestriction (pulsed-field gel electrophoresis) patterns of were observed in the carcasses before and after chilling. Pork samples from treated and untreated carcasses with ozone showed no lipid oxidation or altered color and pH. The results indicate that the gaseous ozone in the tested protocol is effective in reducing TAM populations, but not effective in decreasing the number of carcasses positive for and sp. Regarding , the tendency of positive carcasses reduction may encourage further studies by testing other protocols of gaseous ozone application inside the chilling chamber.
臭氧处理已被提议作为卫生计划下屠宰动物的附加措施。在这项研究中，我们确定了在冷却过程中（2-5°C 下冷却 16 小时）向猪胴体施加气态臭氧的功效。将 40 个胴体分配到每个处理中：对照组，不施加臭氧（T1）和 5ppm 气态臭氧处理（T2），分为两个 4 小时期。胴体在冷却前后进行采样。冷却前 TAM 总需氧嗜温菌（TAM）的平均计数在 T1 和 T2 之间没有差异（p=0.55）。相反，冷却后，臭氧处理的胴体的 TAM 计数减少了约 0.4 个菌落形成单位（CFU）/cm（p<0.001），而对照组胴体则没有显著减少。在气态臭氧处理后， sp. 和 的阳性胴体数量没有明显减少；而 T2 中 的阳性胴体数量有减少的趋势（p=0.08）。在冷却前后，观察到 sp. 的常见宏限制（脉冲场凝胶电泳）模式。来自经臭氧处理和未经处理的胴体的猪肉样本没有显示出脂质氧化或颜色和 pH 值改变。结果表明，在测试方案中，气态臭氧可有效降低 TAM 种群数量，但不能有效降低 sp. 和 阳性胴体的数量。关于 sp.，阳性胴体数量减少的趋势可能鼓励进一步研究，通过测试冷却室内其他气态臭氧应用方案。
