CAS Key Laboratory of Bio-Inspired Materials and Interface Sciences, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Chemistry, Beihang University, Beijing, 100191, P. R. China.
Adv Mater. 2021 Jun;33(25):e2003559. doi: 10.1002/adma.202003559. Epub 2021 May 13.
Unwanted protein adsorption deteriorates fouling processes and reduces analytical device performance. Wettability plays an important role in protein adsorption by affecting interactions between proteins and surfaces. However, the principles of protein adsorption are not completely understood, and surface coatings that exhibit resistance to protein adsorption and long-term stability still need to be developed. Here, a nanostructured superamphiphilic TiO composite (TiO /SiO ) coating that can effectively prevent nonspecific protein adsorption on water/solid interfaces is reported. The confined water on the superamphiphilic surface enables a low adhesion force and the formation of an energy barrier that plays a key role in preventing protein adsorption. This adaptive design protects the capillary wall from fouling in a harsh environment during the bioanalysis of capillary electrophoresis and is further extended to applications in multifunctional microfluidics for liquid transportation. This facile approach is not only perfectly applied in channels with complicated configurations but may also offer significant insights into the design of advanced superwetting materials to control biomolecule adhesion in biomedical devices, microfluidics, and biological assays.
非期望蛋白的吸附会恶化污垢形成过程，并降低分析器件的性能。润湿性通过影响蛋白质与表面之间的相互作用，在蛋白质吸附中起着重要作用。然而，蛋白质吸附的原理还不完全清楚，仍然需要开发表现出抗蛋白质吸附和长期稳定性的表面涂层。在此，报道了一种纳米结构的超双疏 TiO 复合（TiO /SiO ）涂层，它可以有效地防止水/固界面上非特异性蛋白质的吸附。超双疏表面上的受限水可产生低粘附力，并形成阻止蛋白质吸附的能量势垒，这在阻止蛋白质吸附方面起着关键作用。这种自适应设计可以保护毛细管壁在毛细管电泳的生物分析过程中免受恶劣环境的污染，并进一步扩展到用于液体输送的多功能微流控应用中。这种简单的方法不仅可以完美地应用于具有复杂结构的通道，而且可能为设计先进的超润湿材料以控制生物医学设备、微流控和生物分析中的生物分子粘附提供重要的见解。
