Nikolova Maria P, Tzvetkov Iliyan, Dimitrova Tanya V, Ivanova Veronika L, Handzhiyski Yordan, Andreeva Andreana, Valkov Stefan, Ormanova Maria, Apostolova Margarita D
Department of Material Science and Technology, University of Ruse "Angel Kanchev", 8 Studentska Str., 7017 Ruse, Bulgaria.
Roumen Tsanev Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
Nanomaterials (Basel). 2024 Jul 4;14(13):1148. doi: 10.3390/nano14131148.
One of the primary risk factors for implant failure is thought to be implant-related infections during the early healing phase. Developing coatings with cell stimulatory behaviour and bacterial adhesion control is still difficult for bone implants. This study proposes an approach for one-step deposition of biocompatible and antimicrobial Cu-doped TiO coatings via glow-discharge sputtering of a mosaic target. During the deposition, the bias of the Ti6Al4V substrates was changed. Structure examination, phase analysis, and surface morphology were carried out using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The hardness values and hydrophilic and corrosion performance were also evaluated together with cytocompatible and antibacterial examinations against and . The results show great chemical and phase control of the bias identifying rutile, anatase, CuO, or ternary oxide phases. It was found that by increasing the substrate bias from 0 to -50 V the Cu content increased from 15.3 up to 20.7 at% while at a high bias of -100 V, the copper content reduced to 3 at%. Simultaneously, apart from the Cu state, Cu is also found in the biased samples. Compared with the bare alloy, the hardness, the water contact angle and corrosion resistance of the biased coatings increased. According to an assessment of in vitro cytocompatibility, all coatings were found to be nontoxic to MG-63 osteoblast cells over the time studied. Copper release and cell-surface interactions generated an antibacterial effect against and strains. The -50 V biased coating combined the most successful results in inhibiting bacterial growth and eliciting the proper responses from osteoblastic cells because of its phase composition, electrochemical stability, hydrophilicity, improved substrate adhesion, and surface roughness. Using this novel surface modification approach, we achieved multifunctionality through controlled copper content and oxide phase composition in the sputtered films.
种植体失败的主要风险因素之一被认为是早期愈合阶段与种植体相关的感染。对于骨植入物而言，开发具有细胞刺激行为和细菌粘附控制功能的涂层仍然具有挑战性。本研究提出了一种通过镶嵌靶材的辉光放电溅射一步沉积生物相容性和抗菌性铜掺杂二氧化钛涂层的方法。在沉积过程中，改变了Ti6Al4V基底的偏压。使用X射线衍射（XRD）分析、扫描电子显微镜（SEM）、原子力显微镜（AFM）和X射线光电子能谱（XPS）进行结构检查、相分析和表面形貌分析。还评估了硬度值、亲水性和耐腐蚀性能，以及针对 和 的细胞相容性和抗菌性检查。结果表明，通过偏压可以很好地控制化学和相组成，确定金红石、锐钛矿、CuO或三元氧化物相。研究发现，将基底偏压从0 V增加到-50 V时，铜含量从15.3 at%增加到20.7 at%，而在-100 V的高偏压下，铜含量降至3 at%。同时，除了铜的状态外，在有偏压的样品中也发现了铜。与裸合金相比，有偏压涂层的硬度、水接触角和耐腐蚀性有所增加。根据体外细胞相容性评估，在所研究的时间段内，所有涂层对MG-63成骨细胞均无毒。铜的释放和细胞表面相互作用对 和 菌株产生了抗菌作用。-50 V偏压涂层在抑制细菌生长和引发成骨细胞的适当反应方面结合了最成功的结果，这归因于其相组成、电化学稳定性、亲水性、改善的基底附着力和表面粗糙度。通过这种新颖的表面改性方法，我们通过控制溅射薄膜中的铜含量和氧化物相组成实现了多功能性。
