School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou 510006, China.
Fitoterapia. 2021 Jul;152:104924. doi: 10.1016/j.fitote.2021.104924. Epub 2021 May 11.
Lychee is a favorite fruit of the Cantonese and native to Southeast Asia. In this study, the anti-neuroinflammatory bioactive compounds of lychee seeds have been carried out. Five new jasmonates (1, 2, 6-8) and seventeen known compounds were isolated using a series of chemical and chromatographic methods. Their chemical structures were identified through comprehensive spectroscopic analysis. Anti-neuroinflammatory activities were assayed and evaluated for the purified compounds. Most of the compounds exhibited pronounced anti-neuroinflammatory activities on nitric oxide (NO) induced by lipopolysaccharide (LPS) in BV-2 microglia cells. Moreover, compounds 1, 2 and 20 could reduce the expression of LPS-induced pro-inflammatory factors (iNOS and COX-2), inhibit the expression of mRNA levels of iNOS, COX-2, IL-6 and block NF-κB nuclear translocation in dose-dependent manners. This study suggested that lychee phytochemicals could be benefit to some neuroinflammatory-associated diseases, such as Alzheimer's disease.
荔枝是广东人和东南亚地区的人喜爱的水果。在这项研究中，对荔枝种子的抗神经炎症生物活性化合物进行了研究。采用一系列化学和色谱方法，从荔枝种子中分离得到了五个新的茉莉酸（1、2、6-8）和十七个已知化合物。通过综合光谱分析确定了它们的化学结构。对分离得到的化合物进行了抗神经炎症活性测定和评价。大多数化合物对脂多糖（LPS）诱导的BV-2 小胶质细胞一氧化氮（NO）表现出显著的抗神经炎症活性。此外，化合物 1、2 和 20 可以降低 LPS 诱导的促炎因子（iNOS 和 COX-2）的表达，抑制 iNOS、COX-2、IL-6 的 mRNA 水平的表达，并呈剂量依赖性阻断 NF-κB 核转位。本研究表明，荔枝植物化学物质可能对一些与神经炎症相关的疾病有益，如阿尔茨海默病。
