Department of Neurochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Biochem Biophys Res Commun. 2021 Jun 30;560:105-111. doi: 10.1016/j.bbrc.2021.04.116. Epub 2021 May 10.
Anti-prion effects of cellulose ether (CE) are reported in rodents, but the molecular mechanism is fully unknown. Here, we investigated the genetic background of CE effectiveness by proteomic and genetic analysis in mice. Proteomic analysis in the two mouse lines showing a dramatic difference in CE effectiveness revealed a distinct polymorphism in the glia maturation factor β gene. This polymorphism was significantly associated with the CE effectiveness in various prion-infected mouse lines. Sequencing of this gene and its vicinity genes also revealed several other polymorphisms that were significantly related to the CE effectiveness. These polymorphisms are useful as genetic markers for finding more suitable mouse lines and exploring the genetic factors of CE effectiveness.
已有研究报道纤维素醚（CE）具有抗朊病毒的作用，但具体的分子机制尚不清楚。在这里，我们通过对具有显著不同 CE 效果的两种小鼠进行蛋白质组学和遗传学分析，来研究 CE 效果的遗传背景。在这两种显示出明显差异的小鼠品系中进行蛋白质组学分析，揭示了神经胶质细胞成熟因子β基因中的一个独特多态性。该多态性与各种朊病毒感染的小鼠品系中的 CE 效果显著相关。对该基因及其附近基因的测序还揭示了几个与 CE 效果显著相关的其他多态性。这些多态性可用作遗传标记，以找到更合适的小鼠品系，并探索 CE 效果的遗传因素。
