College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, 430065, China.
College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, 430065, China; School of Materials and Metallurgy, Wuhan University of Science and Technology, Wuhan, 430081, China.
Biochem Biophys Res Commun. 2021 Jun 30;560:112-118. doi: 10.1016/j.bbrc.2021.04.091. Epub 2021 May 10.
RNA polymerase I (Pol I) is responsible for the synthesis of the majority of ribosomal RNA molecules in eukaryotes. Pol I subunit 12 (RPA12) is involved in the transcriptional termination and lipid metabolism in yeast. However, its role in human cells hasn't been investigated so far. Here, we show that RPA12 is present in the nucleolus and nucleoplasm of HeLa cells. RPA12 can act as a positive factor to regulate Pol I-mediated transcription and the proliferation of 293T and HeLa cells. Unexpectedly, RPA12 can repress HeLa cell migration, indicating that RPA12 plays opposite roles in cell proliferation and migration. This study provides a novel insight into the role of RPA12 in human cells.
RNA 聚合酶 I（Pol I）负责真核生物中大多数核糖体 RNA 分子的合成。Pol I 亚基 12（RPA12）参与酵母中的转录终止和脂质代谢。然而，迄今为止，其在人细胞中的作用尚未被研究。在这里，我们表明 RPA12 存在于 HeLa 细胞的核仁中和核质中。RPA12 可以作为一个正因子来调节 Pol I 介导的转录以及 293T 和 HeLa 细胞的增殖。出乎意料的是，RPA12 可以抑制 HeLa 细胞的迁移，表明 RPA12 在细胞增殖和迁移中发挥相反的作用。本研究为 RPA12 在人细胞中的作用提供了新的见解。
