Anal Methods. 2013 Dec 19;6(2):333-336. doi: 10.1039/c3ay90101g.
DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied. Mullis's invention allowed researchers to make millions of copies of a selected DNA region within hours. Today, PCR can take minutes. PCR has widespread analytical applications in the food, environmental, medical and forensic fields. This Technical Brief covers the basics of PCR and some of its variations.
在分析实验室中，基于DNA的检测方法正变得越来越普遍，其中聚合酶链式反应（PCR）已成为一项不可或缺的技术。PCR由卡里·B·穆利斯于1985年发明，它彻底改变了脱氧核糖核酸（DNA）的复制方式。穆利斯的发明使研究人员能够在数小时内复制数百万份选定的DNA区域。如今，PCR只需几分钟。PCR在食品、环境、医学和法医领域有着广泛的分析应用。本技术简报涵盖了PCR的基本原理及其一些变体。
