实时 PCR：基因检测和表达分析的革命。
Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.
机构信息
Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, India.
出版信息
Curr Genomics. 2007 Jun;8(4):234-51. doi: 10.2174/138920207781386960.
Abstract
Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.
摘要
聚合酶链反应（PCR）技术由 Kary Mullis 于 1984 年发明，催生了实时 PCR。实时 PCR——实时检测和表达分析基因——由于其在定量基因分型、生物体内外遗传变异、疾病早期诊断、法医学等方面的巨大应用，彻底改变了 21 世纪的生物科学。我们全面回顾了实时 PCR 的各个方面，包括技术改进以及在从医学到环境问题再到植物等各个科学领域的应用。
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Plant Dis. 1999 Dec;83(12):1095-1100. doi: 10.1094/PDIS.1999.83.12.1095.
2
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