Morley D, Santamore W P
Department of Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
J Pharmacol Methods. 1988 Jul;19(4):319-38. doi: 10.1016/0160-5402(88)90006-x.
Clinical and experimental evidence suggest a strong relationship between arterial stenosis, platelet aggregation, and subsequent thrombus formation. To facilitate the study of platelet accumulation in stenotic arteries, we developed an in vitro preparation. Arterial segments were perfused with whole citrated blood. A stenosis was created by applying an external plastic constrictor to the artery. Platelet accumulation within the stenosis was assessed by scanning electron microscopy and by radioactive counts from Indium-111 labeled platelets. Utilizing this preparation, 30 carotid arterial segments from 10 mongrel dogs were perfused at 100 mmHg for 15 min. In 10 arteries without a stenosis, scanning electron microscopy and radioactive counts demonstrated little platelet accumulation. In contrast, extensive platelet aggregation was observed in 10 arteries with stenoses. Moreover, in 10 stenotic arteries exposed to the thromboxane mimetic, U46619 (Upjohn Diagnostic Group), scanning electron microscopy and radioactive counts demonstrated a significant increase in platelet deposition. Conversely, we demonstrated a dimunition of platelet accumulation in stenosed arterial segments exposed to the prostacyclin analogue platelet inhibitor, Iloprost (Berlex Laboratories, Inc.). The in vitro preparation allows precise control of hemodynamic variables and makes it possible to perform multiple tests on segments of the same vessel from the same animal.
临床和实验证据表明，动脉狭窄、血小板聚集与随后的血栓形成之间存在密切关系。为便于研究血小板在狭窄动脉中的聚集情况，我们开发了一种体外制备方法。用枸橼酸化全血灌注动脉段。通过对动脉施加外部塑料收缩器来制造狭窄。通过扫描电子显微镜和来自铟-111标记血小板的放射性计数来评估狭窄部位的血小板聚集情况。利用这种制备方法，对10只杂种犬的30个颈动脉段在100 mmHg压力下灌注15分钟。在10条无狭窄的动脉中，扫描电子显微镜和放射性计数显示血小板聚集很少。相比之下，在10条有狭窄的动脉中观察到广泛的血小板聚集。此外，在10条暴露于血栓素类似物U46619（Upjohn诊断集团）的狭窄动脉中，扫描电子显微镜和放射性计数显示血小板沉积显著增加。相反，我们证明在暴露于前列环素类似物血小板抑制剂伊洛前列素（贝林实验室公司）的狭窄动脉段中，血小板聚集减少。这种体外制备方法允许精确控制血流动力学变量，并使得对来自同一动物的同一血管段进行多项测试成为可能。
