Laboratory of Genetic Toxicology, Postgraduate Program in Molecular and Cell Biology Applied to Health (PPGBioSaúde), Lutheran University of Brazil (ULBRA), Avenida Farroupilha, 8001 Bairro São José, CEP 92425-900, Canoas, RS, Brazil.
Laboratory of Genetic Toxicology, Postgraduate Program in Molecular and Cell Biology Applied to Health (PPGBioSaúde), Lutheran University of Brazil (ULBRA), Avenida Farroupilha, 8001 Bairro São José, CEP 92425-900, Canoas, RS, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2021 Jun;866:503350. doi: 10.1016/j.mrgentox.2021.503350. Epub 2021 Mar 18.
Coal burning generates gases, particles, and condensation by-products that are harmful to soil, water, and to the atmosphere. The aim of this study was to characterize and identify the cytotoxic and mutagenic potential of soil samples from the cities of Aceguá, Bagé, Candiota and Pinheiro Machado, near a large coal-fired power plant. Our study describes soil characteristics and contributes to the evaluation of the genotoxic activity of coal mining and burning, using the Comet Assay and Micronucleus test in V79 cells, as well as mutagenicity assays with Salmonella typhimurium strains. Comet Assay results show that the winter soil samples of Candiota and Pinheiro Machado induced a significant increase of the Damage Index for cells, as well as for the Aceguá summer sample. The micronucleus test did not detect differences between cities and seasons. A component analysis indicates associations between results obtained in Comet Assay and Ti and phenanthene concentrations for Pinheiro Machado during the winter, and Al for Aceguá during the summer and Zn during the winter. Results of Salmonella/microsome assays were negative, only Candiota and Pinheiro Machado samples showed a statistical increase of his + colonies in TA102. Our work describes biological data on these cells exposed to coal-contaminated soil, confirming the sensitivity of the Comet Assay in V79 cells and Salmonella/microsome assay for the evaluation of the effects of complex mixtures. These findings help to understand the spatial distribution of contaminants in the local soil related to a power plant, which is important for planning public safety actions.
燃煤产生的气体、颗粒和冷凝副产品会对土壤、水和大气造成危害。本研究的目的是对阿塞瓜、巴热、坎迪奥塔和皮涅罗·马查多等城市靠近大型燃煤电厂的土壤样本进行特征描述和鉴定，以评估其细胞毒性和致突变潜力。我们的研究描述了土壤特征，并通过 Comet 分析和 V79 细胞微核试验评估了采煤和燃煤的遗传毒性活性，同时还进行了沙门氏菌突变试验。Comet 分析结果表明，坎迪奥塔和皮涅罗·马查多冬季土壤样本的细胞损伤指数显著增加，阿塞瓜夏季样本也出现了这种情况。微核试验未检测到不同城市和季节之间的差异。成分分析表明，Comet 分析结果与皮涅罗·马查多冬季的 Ti 和菲浓度以及阿塞瓜夏季和冬季的 Al 之间存在关联。沙门氏菌/微粒体试验结果为阴性，仅坎迪奥塔和皮涅罗·马查多样本在 TA102 中显示出 his + 菌落的统计增加。我们的工作描述了这些细胞暴露于受煤污染土壤后的生物学数据，证实了 Comet 分析在 V79 细胞和沙门氏菌/微粒体试验中对评估复杂混合物影响的敏感性。这些发现有助于了解与电厂相关的当地土壤中污染物的空间分布，这对于规划公共安全措施非常重要。
