Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia.
Department of Pathology, School of Medicine, University of Otago, Dunedin, New Zealand.
Sci Rep. 2021 May 13;11(1):10278. doi: 10.1038/s41598-021-89740-0.
Breast cancer is the most common cancer that causes death in women. Conventional therapies, including surgery and chemotherapy, have different therapeutic effects and are commonly associated with risks and side effects. Near infrared radiation is a technique with few side effects that is used for local hyperthermia, typically as an adjuvant to other cancer therapies. The understanding of the use of near NIR as a monotherapy, and its effects on the immune cells activation and infiltration, are limited. In this study, we investigate the effects of HT treatment using NIR on tumor regression and on the immune cells and molecules in breast tumors. Results from this study demonstrated that local HT by NIR at 43 °C reduced tumor progression and significantly increased the median survival of tumor-bearing mice. Immunohistochemical analysis revealed a significant reduction in cells proliferation in treated tumor, which was accompanied by an abundance of heat shock protein 70 (Hsp70). Increased numbers of activated dendritic cells were observed in the draining lymph nodes of the mice, along with infiltration of T cells, NK cells and B cells into the tumor. In contrast, tumor-infiltrated regulatory T cells were largely diminished from the tumor. In addition, higher IFN-γ and IL-2 secretion was observed in tumor of treated mice. Overall, results from this present study extends the understanding of using local HT by NIR to stimulate a favourable immune response against breast cancer.
乳腺癌是导致女性死亡的最常见癌症。传统疗法，包括手术和化疗，具有不同的治疗效果，通常与风险和副作用有关。近红外辐射是一种副作用较小的技术，用于局部热疗，通常作为其他癌症治疗的辅助手段。对于近 NIR 作为单一疗法的应用及其对免疫细胞激活和浸润的影响，人们的了解有限。在这项研究中，我们研究了使用 NIR 的 HT 治疗对肿瘤消退以及乳腺肿瘤中免疫细胞和分子的影响。研究结果表明，43°C 的 NIR 局部 HT 可减少肿瘤进展，并显著延长荷瘤小鼠的中位生存时间。免疫组织化学分析显示，治疗后肿瘤细胞增殖显著减少，同时伴有热休克蛋白 70（Hsp70）的大量增加。在小鼠的引流淋巴结中观察到活化的树突状细胞数量增加，同时 T 细胞、NK 细胞和 B 细胞浸润到肿瘤中。相比之下，肿瘤浸润的调节性 T 细胞从肿瘤中大量减少。此外，在治疗小鼠的肿瘤中观察到更高水平的 IFN-γ和 IL-2 分泌。总的来说，本研究的结果扩展了使用 NIR 进行局部 HT 以刺激针对乳腺癌的有利免疫反应的理解。
