Brode E, Kripp U, Breckwoldt W
Knoll AG, Dept. Biochemistry, Ludwigshafen/Rhein, FRG.
Methods Find Exp Clin Pharmacol. 1988 May;10(5):319-29.
A high pressure liquid chromatographic (HPLC) method with internal analogue standardization for the determination of 5-hydroxypropafenone in plasma is described. The method comprises extraction from plasma at pH 9 with diethyl-ether and quantification after HPLC separation using a reverse phase by means of electrochemical (reductive) detection after electrochemical preoxidation. When using 1 ml plasma the lower limit of detection is 0.5 ng/ml. Under routine conditions the limit of determination is estimated to be lower than 2 ng/ml. The variation coefficients of duplicates drop from about 10% in the range of the determination limit to about 3% at 5 ng/ml and above. The determination of 5-hydroxypropafenone is not impaired by other known metabolites of propafenone.
描述了一种采用内标标准化的高压液相色谱（HPLC）法测定血浆中5-羟基普罗帕酮的方法。该方法包括在pH 9条件下用乙醚从血浆中萃取，并在HPLC分离后，采用反相电化学（还原）检测法进行定量，该检测法需先进行电化学预氧化。当使用1 ml血浆时，检测下限为0.5 ng/ml。在常规条件下，测定限估计低于2 ng/ml。重复测定的变异系数从测定限范围内的约10%降至5 ng/ml及以上时的约3%。普罗帕酮的其他已知代谢物不影响5-羟基普罗帕酮的测定。
