An Xuejiao, Zhang Shengwei, Li Taotao, Chen Nana, Wang Xia, Zhang Baojun, Ma Youji
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
Gansu Provincial Farmer Education and Training Station, Lanzhou, China.
PeerJ. 2021 Apr 27;9:e11295. doi: 10.7717/peerj.11295. eCollection 2021.
To date, utilization of feed grains is increasing, which competes for human food. It is imperative to develop and utilize unconventional feed materials. . () is a good feeding material with high crude protein, crude fat, and low crude fiber, which is widely distributed in China. In this study, 12 Dorper ♂×Hu ♀ crossbred weaned male lambs were seleted into four groups based on the feed that ratio of the fermented feed in the total mixed diet (0%, 6%, 18%, and 100%), to character the lambs' longissimus dorsi (LD) fatty acids, morphology and transcriptome. Results showed that the muscle fiber's diameter and area were the smallest in the 100% group. The highest content of beneficial fatty acids and the lowest content of harmful fatty acids in group 18%. RNA-seq identified 443 differentially expressed genes (DEGs) in the LD of lambs from 4 groups. Among these genes, 169 (38.1%) were up-regulated and 274 (61.9%) were down-regulated. The DEGs were mostly enriched in in fatty acid metabolism, arginine and proline metabolism, and PPAR signaling pathways. Our results provide knowledge to understand effect of different ratios of fermented feed on sheep meat quality traits, also a basis for understanding of the molecular regulation mechanism of fermented feed affecting on sheep meat quality.
迄今为止，饲料谷物的使用量在增加，这与人类食物形成了竞争。开发和利用非常规饲料原料势在必行。（）是一种优质饲料原料，粗蛋白、粗脂肪含量高，粗纤维含量低，在中国分布广泛。本研究选取12只杜泊♂×湖羊♀杂交断奶公羔羊，根据全混合日粮中发酵饲料的比例（0%、6%、18%和100%）分为四组，以表征羔羊背最长肌（LD）脂肪酸、形态和转录组。结果表明，100%组的肌纤维直径和面积最小。18%组有益脂肪酸含量最高，有害脂肪酸含量最低。RNA测序鉴定出四组羔羊背最长肌中有443个差异表达基因（DEG）。其中，169个（38.1%）上调，274个（61.9%）下调。这些差异表达基因主要富集在脂肪酸代谢、精氨酸和脯氨酸代谢以及PPAR信号通路中。我们的研究结果为理解不同比例发酵饲料对羊肉品质性状的影响提供了知识，也为理解发酵饲料影响羊肉品质的分子调控机制提供了依据。
