Faculty of Animal Science, Post-Doctoral Animal Production and Technology Student, IPB University, Bogor, Indonesia.
Faculty of Animal Science, Jambi University, Jambi, Indonesia.
PLoS One. 2024 Sep 9;19(9):e0310336. doi: 10.1371/journal.pone.0310336. eCollection 2024.
The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T>C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T>C CYP2E1 gene and lamb quality traits were significant (P<0.05), including carcass characteristics, retail cut carcass, fatty acid, cholesterol, flavor, and odor. Lambs with the CT genotype had a higher mRNA and protein expression in high lamb quality traits. The highest CYP2E1 protein expression was localized in the longissimus dorsi. The group sample with high lamb quality had a higher area and perimeter of muscle cells. CYP2E1 can be used as a genetic marker for selecting sheep with high meat quality.
CYP2E1 在氧化中的作用对于其对肉质的影响至关重要。本研究使用 200 只印度尼西亚绵羊（Ovis aries）来确定 CYP2E1 基因 SNP g 等位基因频率。g.50658168T>C 位于 3´-UTR 区域，与羔羊品质性状相关，包括胴体特征、零售切块胴体、肉质理化特性、脂肪酸、胆固醇、风味和气味以及矿物质含量。此外，还测定了肌肉中 CYP2E1 mRNA 和 CYP2E1 蛋白的表达水平，并与羔羊品质性状相关联。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析鉴定 CYP2E1 基因多态性。使用定量实时 PCR（qRT-PCR）分析表型差异绵羊群体中 CYP2E1 mRNA 表达水平。使用免疫组织化学（IHC）和苏木精-伊红（HE）染色分析，根据 pH 值和嫩度，在高和低羔羊品质组中各使用三个样本。使用一般线性模型（GLM）分析进行 CYP2E1 基因多态性的关联研究。SNP g.50658168T>C CYP2E1 基因 CC、CT 和 TT 基因型与羔羊品质性状之间存在显著关联（P<0.05），包括胴体特征、零售切块胴体、脂肪酸、胆固醇、风味和气味。CT 基因型的羔羊在高羔羊品质性状中具有更高的 mRNA 和蛋白表达。CYP2E1 蛋白表达最高的部位是背最长肌。具有高羔羊品质的组样本具有更高的肌肉细胞面积和周长。CYP2E1 可作为选择具有高肉质的绵羊的遗传标记。
