Chen Cen, Lu Bingguo, Huang Xiaofang, Bi Chuyun, Zhao Lili, Hu Yunzhuo, Chen Xuanyang, Lin Shiqiang, Huang Kai
Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.
Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
PeerJ. 2021 Apr 27;9:e11354. doi: 10.7717/peerj.11354. eCollection 2021.
Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
在基因克隆过程中，经常需要合并桑格测序序列。在本研究中，我们提供了一个Python脚本，它能够组装多个重叠的桑格测序序列。该脚本利用串联桑格测序序列中的重叠区域来合并这些序列。结果表明，该脚本可以在一次运行中从输入的桑格测序序列生成合并后的序列。该脚本为合并桑格测序序列提供了一种简单且免费的方法，对基因克隆很有用。
