成年自发性高血压大鼠垂体前叶中血管紧张素II结合亲和力和结合能力降低。

Decreased angiotensin II binding affinity and binding capacity in the anterior pituitary gland of adult spontaneously hypertensive rats.

作者信息

Gutkind J S, Castrén E, Saavedra J M

机构信息

Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.

出版信息

Life Sci. 1988;43(5):445-51. doi: 10.1016/0024-3205(88)90524-3.

DOI:10.1016/0024-3205(88)90524-3

PMID:3398707

Abstract

Angiotensin II (ANG) binding sites were quantified in single pituitary glands from 4-week-old and 14-week-old male spontaneously hypertensive rats (SHR) and age-matched male normotensive Wistar-Kyoto (WKY) control rats after incubation with 125I-[Sar1]-ANG, autoradiography with computerized densitometry, and comparison to 125I-standards. The maximum binding capacity (Bmax) decreased while the dissociation constant (Kd) for ANG increased in 14-week-old SHR when compared to age-matched WKY control rats (Bmax: 265 +/- 9 and 224 +/- 4 fmol/mg protein; Kd: 0.79 +/- 0.04 and 1.14 +/- 0.08 10(-9) M in WKY and SHR, respectively). Conversely, no difference between rat strains was found in 4-week-old animals. Our results suggest that pituitary ANG binding sites may play a role in the pathophysiology of established genetic hypertension.

摘要

用125I-[Sar1]-血管紧张素II（ANG）孵育后，通过放射自显影结合计算机密度测定法，并与125I标准品进行比较，对4周龄和14周龄雄性自发性高血压大鼠（SHR）以及年龄匹配的雄性血压正常的Wistar-Kyoto（WKY）对照大鼠的单个垂体中的ANG结合位点进行定量分析。与年龄匹配的WKY对照大鼠相比，14周龄SHR的最大结合容量（Bmax）降低，而ANG的解离常数（Kd）增加（WKY和SHR的Bmax分别为265±9和224±4 fmol/mg蛋白质；Kd分别为0.79±0.04和1.14±0.08×10⁻⁹ M）。相反，在4周龄动物中未发现大鼠品系之间存在差异。我们的结果表明，垂体ANG结合位点可能在遗传性高血压的病理生理学中起作用。