Gutkind J S, Kurihara M, Castren E, Saavedra J M
Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
J Hypertens. 1988 Jan;6(1):79-84. doi: 10.1097/00004872-198801000-00012.
We studied the density of the angiotensin II (Ang II) binding site in discrete brain nuclei of 4-week-old and 14-week-old spontaneously hypertensive rats (SHR) and age-matched normotensive Wistar-Kyoto (WKY) control rats by autoradiographic binding techniques. Tissue sections were incubated in vitro with 3 nmol/l [125I]Sar1Ang and results were analysed by computerized microdensitometry and by comparison with 125I-standards. Both young and adult SHR (aged 4 and 14 weeks, respectively) had significantly higher Ang II binding site concentrations in the median preoptic nucleus (MPO), subfornical organ (SFO), paraventricular nucleus (PVN) and nucleus of the solitary tract (NTS) when compared to age-matched WKY control rats. No significant difference was found between strains in other brain areas such as the olfactory bulb, suprachiasmatic nucleus (SCh), inferior olive (IO) and area postrema (AP). It was observed that the concentration of Ang II binding sites increased with age in PVN of both SHR and WKY, while the number of binding sites in the MPO and IO decreased with age. In SHR, alteration in Ang II binding is restricted to brain nuclei involved in the central pressor action of Ang II and seems to be related to the development and maintenance of spontaneous hypertension.
我们采用放射自显影结合技术，研究了4周龄和14周龄自发性高血压大鼠（SHR）以及年龄匹配的正常血压Wistar-Kyoto（WKY）对照大鼠离散脑核中血管紧张素II（Ang II）结合位点的密度。将组织切片与3 nmol/l [125I]Sar1Ang进行体外孵育，结果通过计算机微密度测定法并与125I标准品进行比较来分析。与年龄匹配的WKY对照大鼠相比，年轻和成年SHR（分别为4周龄和14周龄）在视前正中核（MPO）、穹窿下器官（SFO）、室旁核（PVN）和孤束核（NTS）中的Ang II结合位点浓度显著更高。在其他脑区，如嗅球、视交叉上核（SCh）、下橄榄核（IO）和最后区（AP），各品系之间未发现显著差异。观察到，SHR和WKY的PVN中Ang II结合位点的浓度均随年龄增加，而MPO和IO中的结合位点数随年龄减少。在SHR中，Ang II结合的改变仅限于参与Ang II中枢升压作用的脑核，并且似乎与自发性高血压的发生和维持有关。
