Ahn Jun-Sang, Chung Ki-Yong, Jang Sun-Sick, Kim Ui-Hyung, Hwang So-Mi, Jin Shil, Park Bo-Hye, Kang Dong-Hun, Kwon Eung-Gi
Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Korea.
Department of Beef Science, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea.
J Anim Sci Technol. 2020 Nov;62(6):893-902. doi: 10.5187/jast.2020.62.6.893. Epub 2020 Nov 30.
This study was conducted to compare the mRNA expression levels of myogenic-adipogenic makers in the skeletal muscle and adipocytes formation, body weight, rumen weight, and papilla length on Hanwoo calves at newborn and 6 months of age. Animals used three newborn Hanwoo calves (NC) and three Hanwoo calves 6 months of age (SC). Body weight and rumen weight were significantly increased in SC compared to NC ( < 0.01), and papilla length was longer about 10-fold in SC than NC. Adipocytes was possible to visually identify more adipocytes in SC compared to NC, and were mainly formed around the blood vessels. mRNA expression of myogenin, myosin heavy chain 1 and myosin heavy chain 2A in both (LD) and (SM) was found to increase with calves growth ( < 0.01), and it was confirmed that have higher levels of mRNA expression in SM than LD. In LD tissues, the mRNA expression of stearoyl-CoA desaturase (SCD, < 0.03) and peroxisome proliferator activated receptor γ (PPARγ, < 0.04) was significantly higher in SC than NC. In SM tissues, mRNA expression levels of SCD ( < 0.02) and CCAAT/enhancer binding protein β (C/EBPβ, < 0.01) were higher in SC than NC, and also mRNA expression levels of PPARγ increased, but there was no significant difference. Thus, the calves period suggests that it is an important step in the development of the rumen and the myogenesis and adipogenesis.
本研究旨在比较韩牛犊牛在新生期和6月龄时骨骼肌和脂肪细胞形成过程中肌源性-脂肪源性标志物的mRNA表达水平、体重、瘤胃重量和乳头长度。实验动物为3头新生韩牛犊牛（NC）和3头6月龄韩牛犊牛（SC）。与NC相比，SC的体重和瘤胃重量显著增加（<0.01），SC的乳头长度比NC长约10倍。与NC相比，在SC中可以肉眼观察到更多的脂肪细胞，且主要在血管周围形成。发现长头肌（LD）和半膜肌（SM）中肌生成素、肌球蛋白重链1和肌球蛋白重链2A的mRNA表达随犊牛生长而增加（<0.01），且证实SM中的mRNA表达水平高于LD。在LD组织中，SC中硬脂酰辅酶A去饱和酶（SCD，<0.03）和过氧化物酶体增殖物激活受体γ（PPARγ，<0.04）的mRNA表达显著高于NC。在SM组织中，SC中SCD（<0.02）和CCAAT/增强子结合蛋白β（C/EBPβ，<0.01）的mRNA表达水平高于NC，PPARγ的mRNA表达水平也有所增加，但无显著差异。因此，犊牛期是瘤胃发育以及肌生成和脂肪生成的重要阶段。
