Lee Da Young, Lee Seung Yun, Kang Hea Jin, Park Yeonhwa, Hur Sun Jin
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA.
J Anim Sci Technol. 2020 Nov;62(6):933-947. doi: 10.5187/jast.2020.62.6.933. Epub 2020 Nov 30.
This study was conducted to develop an effective heparin extraction method by using low-cost and highly effective enzymes from six pig by-products (liver, lung, heart, stomach, small intestine, and large intestine), and analyze their bioavailability. Low-cost and highly effective enzymes (alkaline-AK and papain) and a common enzyme (trypsin) were used for the heparin extraction. The angiotensin I - converting enzyme (ACE) inhibitory activity and the antimicrobial activity of extracted heparin were analyzed to verify their bioavailability. The average amount of heparin extracted per kilogram of pig by-products was 439 mg from the liver, 127 mg from the lung, 398 mg from the heart, 261 mg from the stomach, 197 mg from the small intestine, and 239 mg from the large intestine. Various enzymes were used to extract heparin, and the amount of extracted heparin was similar. Based on 1 g of pig by-product, the enzymes trypsin, papain, and alkaline-AK could extract 1,718 mg, 1,697 mg, and 1,905 mg of heparin, respectively. Heparin extracted from pig by-products showed antihypertensive activity and antimicrobial activity against Staphylococcus aureus at low populations. These results indicated that heparin can be obtained from pig by-products at a low cost.
本研究旨在利用来自猪的六种副产品（肝脏、肺、心脏、胃、小肠和大肠）的低成本高效酶开发一种有效的肝素提取方法，并分析其生物利用度。使用低成本高效酶（碱性-AK和木瓜蛋白酶）和一种常用酶（胰蛋白酶）进行肝素提取。分析提取的肝素的血管紧张素I转换酶（ACE）抑制活性和抗菌活性，以验证其生物利用度。每千克猪副产品提取的肝素平均量分别为：肝脏439毫克、肺127毫克、心脏398毫克、胃261毫克、小肠197毫克、大肠239毫克。使用各种酶提取肝素，提取的肝素量相似。基于1克猪副产品，胰蛋白酶、木瓜蛋白酶和碱性-AK分别可提取1718毫克、1697毫克和1905毫克肝素。从猪副产品中提取的肝素在低浓度下显示出抗高血压活性和对金黄色葡萄球菌的抗菌活性。这些结果表明，可以低成本从猪副产品中获得肝素。
