Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi 17546, Republic of Korea.
Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi 17546, Republic of Korea.
Biomed Pharmacother. 2019 Aug;116:109046. doi: 10.1016/j.biopha.2019.109046. Epub 2019 Jun 4.
This study was conducted to purify the angiotensin converting enzyme (ACE) inhibitory peptides from beef myofibrillar proteins by using inexpensive enzymes alkaline-AK and papain. Different molecular weight peptides (<3 and <10 kDa) were obtained using ultrafiltration. The <3 kDa peptides obtained by alkaline-AK (AK3K) digestion showed the highest ACE inhibitory activity (74.29%) as compared to other alkaline-AK peptides, and a strong antihypertensive effect of AK3K was observed in the spontaneously hypertensive rat (SHR) model. The AK3K treatment groups (400 and 800 mg/kg body weight) exhibited a decrease in systolic blood pressure (SBP) by 28 and 35 mmHg, respectively in the SHR model. The study demonstrated that the ACE inhibitory peptide obtained from beef myofibrillar proteins had the sequence Leu-Ile-Val-Gly-Ile-Ile-Arg-Cys-Val, and could be possibly used for lowering the SBP.
本研究旨在利用廉价的碱性蛋白酶 AK 和木瓜蛋白酶从牛肉肌原纤维蛋白中纯化血管紧张素转化酶（ACE）抑制肽。通过超滤获得不同分子量的肽（<3 和 <10 kDa）。与其他碱性 AK 肽相比，碱性 AK 消化获得的<3 kDa 肽（AK3K）显示出最高的 ACE 抑制活性（74.29%），并且在自发性高血压大鼠（SHR）模型中观察到 AK3K 的强烈降压作用。AK3K 治疗组（400 和 800 mg/kg 体重）分别使 SHR 模型中的收缩压（SBP）降低 28 和 35 mmHg。研究表明，从牛肉肌原纤维蛋白中获得的 ACE 抑制肽的序列为 Leu-Ile-Val-Gly-Ile-Ile-Arg-Cys-Val，可能可用于降低 SBP。
