Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China.
Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital, Central South University, Changsha, China.
J Oral Pathol Med. 2021 Nov;50(10):995-1004. doi: 10.1111/jop.13199. Epub 2021 May 27.
G3BP1 is a prognostic biomarker for many types of cancers; however, its role in oral squamous cell carcinoma remains unclear. We investigated the role of G3BP1 as a potential biomarker for proliferation, apoptosis, and prognosis in oral squamous cell carcinoma.
We obtained samples of normal oral mucosa (n = 17), oral squamous cell carcinoma tissues (n = 61), and paired adjacent tissues (n = 47) from Xiangya Hospital for immunohistochemical evaluation to measure the expression of G3BP1, E-cadherin, Ki67, and Cleaved-caspase3. Using data from The Cancer Genome Atlas, we performed bioinformatics analysis to investigate the prognosis, functions, signaling pathways, and immune infiltrate significance related to G3BP1 in oral squamous cell carcinoma.
The G3BP1 protein level was significantly upregulated in oral squamous cell carcinoma tissues and was also positively associated with Ki67 and negatively associated with Cleaved-caspase3. Based on information available in online database, the G3BP1 mRNA level was significantly higher in oral squamous cell carcinoma than in normal tissues. High G3BP1 mRNA levels were associated with poor overall survival rates in patients with oral squamous cell carcinoma. Enrichment analysis showed that G3BP1 was involved in the helicase/catalytic/ATPase activity functions and spliceosome/RNA transport/ cell cycle pathways. Furthermore, G3BP1 mRNA levels were positively associated with CD4 T-cell infiltration.
G3BP1 may serve as a potential biomarker for proliferation, apoptosis, and prognosis of oral squamous cell carcinoma.
G3BP1 是多种癌症的预后生物标志物；然而，其在口腔鳞状细胞癌中的作用尚不清楚。我们研究了 G3BP1 作为口腔鳞状细胞癌增殖、凋亡和预后的潜在生物标志物的作用。
我们从湘雅医院获得了 17 例正常口腔黏膜、61 例口腔鳞状细胞癌组织和 47 对相邻组织的样本，进行免疫组织化学评估，以测量 G3BP1、E-钙黏蛋白、Ki67 和 Cleaved-caspase3 的表达。利用癌症基因组图谱的数据，我们进行了生物信息学分析，以研究 G3BP1 在口腔鳞状细胞癌中的预后、功能、信号通路和免疫浸润意义。
G3BP1 蛋白水平在口腔鳞状细胞癌组织中显著上调，并且与 Ki67 呈正相关，与 Cleaved-caspase3 呈负相关。基于在线数据库中的信息，口腔鳞状细胞癌中 G3BP1 的 mRNA 水平明显高于正常组织。G3BP1 mRNA 水平高与口腔鳞状细胞癌患者总生存率降低相关。富集分析表明，G3BP1 参与了解旋酶/催化/ATP 酶活性功能和剪接体/RNA 转运/细胞周期途径。此外，G3BP1 mRNA 水平与 CD4 T 细胞浸润呈正相关。
G3BP1 可能作为口腔鳞状细胞癌增殖、凋亡和预后的潜在生物标志物。
