Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Anhui, Hefei, P.R. China.
Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Angui, Hefei, China.
Cryo Letters. 2020 Mar-Apr;41(2):100-105.
Human umbilical cord mesenchymal stem cells (hUMSCs) and hUMSCs-hydrogel constructs have great potential in clinical application. However, research about hypothermic storage of hUMSCs and hUMSCs-hydrogel constructs has not been fully investigated.
To evaluate the cell survival without and with encapsulation (alginate, alginate with PVA) at 4 ºC and 25 ºC, respectively.
Acridine orange/ethidium bromide (AO/EB) staining and fluorescence staining were used to evaluate cell viability during hypothermic storage.
Cells with encapsulation show significantly higher viability than cells without encapsulation at 4 ºC, while there was no obvious difference between viability of cells without and with encapsulation at 25 ºC. In addition, cells encapsulated with alginate and PVA show better viability than cells encapsulated with alginate alone. Moreover, cell viability at 25 ºC was significantly higher than cell viability at 4 ºC.
hUMSCs aggregates and hUMSCs-hydrogel constructs can survive for 7 days by hypothermic storage at 25 ºC, which facilitates the cell delivery and application of hUMSCs and hUMSCs-hydrogel constructs in clinical medicine.
人脐带间充质干细胞（hUMSC）和 hUMSC-水凝胶构建体在临床应用中具有巨大潜力。然而，hUMSC 和 hUMSC-水凝胶构建体的低温保存研究尚未得到充分研究。
分别评估 4°C 和 25°C 下未封装和封装（藻酸盐、藻酸盐与 PVA）的细胞存活率。
吖啶橙/溴化乙锭（AO/EB）染色和荧光染色用于评估低温保存过程中的细胞活力。
在 4°C 下，封装的细胞比未封装的细胞具有明显更高的活力，而在 25°C 下，未封装和封装的细胞之间的活力没有明显差异。此外，与单独使用藻酸盐封装的细胞相比，使用藻酸盐和 PVA 封装的细胞具有更好的活力。此外，25°C 下的细胞活力明显高于 4°C 下的细胞活力。
hUMSC 聚集物和 hUMSC-水凝胶构建体可以在 25°C 下低温保存 7 天，这有利于 hUMSC 和 hUMSC-水凝胶构建体在临床医学中的细胞输送和应用。
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