Tao Qingxia, Wu Cuiying, Li Xinda, Chen Wenjin, Sun Kai, Zhang Peng, Yang Zhijun, Liu Ning, Xu Ruxiang, Xu Tao, Wang Chong
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China.
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Cell Mol Biol (Noisy-le-grand). 2020 May 16;66(2):165-171.
The aim of this study was to investigate the effect of three-dimensional (3D) bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D bio-printed microspheres consisting of HUMSCs were constructed using electrostatic inkjet technique. The parameters used for the synthesis of 3D bio-printed tissue constructs were first optimized. The viability, proliferation and differentiation of 3D cultured HUMSCs were assessed. The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth. The optimized printing parameters were 6 kV voltage, 10 mL/h flow, 15 cm receiving height, and alginate: water ratio of 1:1 mixed at 37 °C. Compared with 2D cultured HUMSCs, the 3D cultured HUMSCs have better viability, proliferation and differentiation ability. The results obtained in this study indicate that 3D bio-printed tissue constructs promote HUMSC viability, proliferation, and neural differentiation in vitro.
本研究旨在探讨由人脐带间充质干细胞（HUMSCs）组成的三维（3D）生物打印构建体对体外细胞活力、增殖和分化的影响。使用静电喷墨技术构建了由HUMSCs组成的功能性3D生物打印微球。首先优化了用于合成3D生物打印组织构建体的参数。评估了3D培养的HUMSCs的活力、增殖和分化情况。扫描电子显微镜（SEM）结果显示，分离的HUMSCs呈现成纤维细胞样的纺锤形贴壁生长。优化的打印参数为6 kV电压、10 mL/h流速、15 cm接收高度以及在37°C下藻酸盐与水的比例为1:1混合。与二维培养的HUMSCs相比，三维培养的HUMSCs具有更好的活力、增殖和分化能力。本研究获得的结果表明，3D生物打印组织构建体可促进体外HUMSC的活力、增殖和神经分化。
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