Santoro C, Mermod N, Andrews P C, Tjian R
Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley.
Nature. 1988 Jul 21;334(6179):218-24. doi: 10.1038/334218a0.
The CTF/NF-I group of cellular DNA binding proteins recognizes the sequence GCCAAT and is implicated in eukaryotic transcription as well as DNA replication. Molecular analysis of human CTF/NF-I complementary DNA clones reveals multiple messenger RNA species containing alternative coding regions, apparently as a result of differential splicing. Expression and functional analysis establish that individual gene products can bind to GCCAAT recognition sites and serve both as promoter-selective transcriptional activators and as initiation factors for DNA replication.
细胞DNA结合蛋白的CTF/NF-I组可识别序列GCCAAT，并参与真核转录以及DNA复制过程。对人类CTF/NF-I互补DNA克隆的分子分析揭示了多种含有可变编码区的信使RNA种类，这显然是选择性剪接的结果。表达和功能分析表明，单个基因产物能够结合GCCAAT识别位点，并兼具启动子选择性转录激活因子和DNA复制起始因子的功能。
