School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
Laboratory of Experimental Animal, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
Environ Toxicol Pharmacol. 2021 Aug;86:103672. doi: 10.1016/j.etap.2021.103672. Epub 2021 May 11.
Clinical studies have shown that Intrahepatic cholestasis is closely related to intestinal injury. The gut-liver axis theory suggests that the intestine and liver are closely related, and that bile acids are important mediators linking the intestine and liver. We compared two cholestasis models: a single injection model that received a single subcutaneous ANIT injection (75 mg/kg), and a multiple subcutaneous injection model that received an injection of ANIT (50 mg/kg) every other day for 2 weeks. We used Transmetil (ademetionine 1,4-butanedisulfonate) to relieve intrahepatic cholestasis in the multiple injection group. In the multiple injection group, we found increased hepatic bile duct hyperplasia, increased fibrosis of the liver, increased small intestine inflammation and oxidative damage, increased harmful bile acids, decreased bile acids transporter levels. After treatment with Transmetil, the liver and gut injuries were relieved. These results suggest that intrahepatic cholestasis can cause disorders of the gut-liver axis.
临床研究表明，肝内胆汁淤积与肠道损伤密切相关。肠肝轴理论表明，肠道和肝脏密切相关，胆汁酸是连接肠道和肝脏的重要介质。我们比较了两种胆汁淤积模型：单次注射模型，接受单次皮下 ANIT 注射（75mg/kg）；和多次皮下注射模型，每隔一天接受 ANIT（50mg/kg）注射 2 周。我们使用 Transmetil（丁二磺酸腺苷蛋氨酸）来缓解多次注射组的肝内胆汁淤积。在多次注射组中，我们发现肝内胆管增生增加，肝脏纤维化增加，小肠炎症和氧化损伤增加，有害胆汁酸增加，胆汁酸转运体水平降低。用 Transmetil 治疗后，肝脏和肠道损伤得到缓解。这些结果表明，肝内胆汁淤积可引起肠肝轴紊乱。
