Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 70457, Taiwan; Department of Neurology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70403, Taiwan.
Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 70457, Taiwan.
Brain Stimul. 2021 Jul-Aug;14(4):771-779. doi: 10.1016/j.brs.2021.04.025. Epub 2021 May 11.
Transcranial direct current stimulation (tDCS) provides a noninvasive polarity-specific constant current to treat epilepsy, through a mechanism possibly involving excitability modulation and neural oscillation.
To determine whether EEG oscillations underlie the interictal spike changes after tDCS in rats with chronic spontaneous seizures.
Rats with kainic acid-induced spontaneous seizures were subjected to cathodal tDCS or sham stimulation for 5 consecutive days. Video-EEG recordings were collected immediately pre- and post-stimulation and for the subsequent 2 weeks following stimulation. The acute pre-post stimulation and subacute follow-up changes of interictal spikes and EEG oscillations in tDCS-treated rats were compared with sham. Ictal EEG with seizure behaviors, hippocampal brain-derived neurotrophic factor (BDNF) protein expression, and mossy fiber sprouting were compared between tDCS and sham rats.
Interictal spike counts were reduced immediately following tDCS with augmented delta and diminished beta and gamma oscillations compared with sham. Cathodal tDCS also enhanced delta oscillations in normal rats. However, increased numbers of interictal spikes with a decrease of delta and theta oscillations were observed in tDCS-treated rats compared with sham during the following 2 weeks after stimulation. Resuming tDCS suppressed the increase of interictal spike activity. In tDCS rats, hippocampal BDNF protein expression was decreased while mossy fiber sprouting did not change compared with sham.
The inverse relationship between the changes of delta oscillation and interictal spikes during tDCS on and off stimulation periods indicates that an enhanced endogenous delta oscillation underlies the tDCS inhibitory effect on epileptic excitability.
经颅直流电刺激（tDCS）通过可能涉及兴奋性调节和神经振荡的机制，提供非侵入性的极性特异性恒定电流来治疗癫痫。
确定在慢性自发性癫痫大鼠中，tDCS 后癫痫发作间期棘波变化是否与 EEG 振荡有关。
对用海人酸诱导的自发性癫痫大鼠进行阴极 tDCS 或假刺激 5 天。在刺激前和刺激后立即以及刺激后 2 周收集视频-EEG 记录。将 tDCS 治疗大鼠的急性刺激前后和亚急性随访中癫痫发作间期棘波和 EEG 振荡的变化与假刺激进行比较。比较 tDCS 和假刺激大鼠的癫痫发作期 EEG 与癫痫发作行为、海马脑源性神经营养因子（BDNF）蛋白表达和苔藓纤维发芽。
与假刺激相比，tDCS 后癫痫发作间期棘波计数减少，δ波增加，β和γ波减少。阴极 tDCS 也增强了正常大鼠的 δ 波振荡。然而，与假刺激相比，在刺激后 2 周内，tDCS 治疗大鼠的癫痫发作间期棘波数量增加，δ 和θ波减少。恢复 tDCS 抑制了癫痫发作间期棘波活动的增加。与假刺激相比，tDCS 大鼠的海马 BDNF 蛋白表达减少，而苔藓纤维发芽没有变化。
在刺激和停止刺激期间，δ 振荡变化与癫痫发作间期棘波之间的反相关关系表明，增强的内源性 δ 振荡是 tDCS 抑制癫痫兴奋性的基础。
