López-Fernández Josu, Dolors Benaiges Maria, Valero Francisco
Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Bioresour Technol. 2021 Aug;334:125233. doi: 10.1016/j.biortech.2021.125233. Epub 2021 May 2.
Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace oil. The presence of surface hydrocarbon chains increased the operational stability of the biocatalysts supported on Purolite® and superparamagnetic particles up to 9 and 2 times, respectively. By contrast, the presence of functional groups had no effect on the initial transesterification rate, which was twice higher with the lipase immobilised onto Purolite®. Also, functionalising Purolite® with epoxide and octadecyl groups led to the highest biodiesel and volumetric productivity. This biocatalyst with other substrates including makauba, jatropha, waste cooking oil, and microbial oil, led to similar initial reaction rates. However, simply raising substrate acidity from 0.5 to 2% increased the operational stability of the biocatalysts 15 times. A synergistic effect between acyl-acceptor concentration and substrate acidity was observed. The transesterification reaction was successfully scaled up to 50 mL.
评估了固定在不同功能化聚甲基丙烯酸酯（普洛利特®）和磁铁矿超顺磁性载体上的米根霉脂肪酶作为用果渣油生产生物柴油的催化剂。表面烃链的存在分别使负载在普洛利特®和超顺磁性颗粒上的生物催化剂的操作稳定性提高了9倍和2倍。相比之下，官能团的存在对初始酯交换速率没有影响，固定在普洛利特®上的脂肪酶的初始酯交换速率高出两倍。此外，用环氧基和十八烷基对普洛利特®进行功能化处理可实现最高的生物柴油产量和体积产率。这种生物催化剂与包括马卡巴油、麻风树油、废食用油和微生物油在内的其他底物反应时，初始反应速率相似。然而，仅将底物酸度从0.5%提高到2%就能使生物催化剂的操作稳定性提高15倍。观察到酰基受体浓度与底物酸度之间存在协同效应。酯交换反应成功放大至50毫升。
