College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2011 Oct;102(20):9810-3. doi: 10.1016/j.biortech.2011.07.070. Epub 2011 Jul 24.
The Rhizopus oryzae lipase containing prosequence was expressed in Pichia pastoris. Recombinant lipase subunit showed a molecular mass of 32 kDa. The maximum activity of recombinant lipase obtained from Mut(s) recombinant was 90 IU/ml. The enzyme was stable in broad ranges of temperatures and pH, with the optimal temperature at 35 °C and pH 7.0. The crude recombinant R. oryzae lipase can be directly used for the transesterification of plant oils at high-water content of 60-100% (w/w) based on oil weight. The addition of 80% water to the transesterification systems resulted in the yield of methyl ester of 95%, 94% and 92% after 72 h using soybean oil, Jatropha curcas seed raw oil and Pistacia chinensis seed raw oil as raw material, respectively. These results indicate that the recombinant lipase is an effective biocatalyst for enzymatic biodiesel production.
米根霉脂肪酶前导序列在毕赤酵母中表达。重组脂肪酶亚基的分子量为 32 kDa。从 Mut(s)重组体中获得的重组脂肪酶的最大活性为 90 IU/ml。该酶在较宽的温度和 pH 范围内稳定，最适温度为 35°C，最适 pH 为 7.0。粗制的重组米根霉脂肪酶可以直接用于在高含水量为 60-100%（基于油重）的条件下进行植物油的酯交换反应。在酯交换体系中添加 80%的水，分别使用大豆油、麻疯树种子原油和黄连木种子原油作为原料，72 h 后甲酯的得率为 95%、94%和 92%。这些结果表明，重组脂肪酶是一种有效的生物催化剂，可用于酶法生物柴油的生产。
