Shatkin-Margolis Abigail, White James, Jedlicka Anne E, Tam Tiffanie, Hill Austin, Yeung Jennifer, Crisp Catrina C, Pauls Rachel N
Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics & Gynecology, Good Samaritan Hospital TriHealth, Cincinnati, OH, USA.
Kaiser Permanente-Dublin Medical Offices & Cancer Center, 3100 Dublin Blvd, Dublin, CA, 94568, USA.
Int Urogynecol J. 2022 Mar;33(3):665-671. doi: 10.1007/s00192-021-04812-z. Epub 2021 May 15.
The objective was to investigate the impact of mindfulness-based stress reduction therapy on the urinary microbiome of patients with interstitial cystitis/bladder pain syndrome.
In this Institutional Review Board-approved prospective cohort study, patients with interstitial cystitis/bladder pain syndrome were recruited to attend an 8-week mindfulness-based stress reduction course involving yoga and meditation. Eligible participants were English-speaking women aged 18 or older with interstitial cystitis/bladder pain syndrome. All participants had a negative urinalysis within 2 months of enrollment and were currently undergoing first- or second-line treatment at the time of recruitment. The mindfulness-based stress reduction course met weekly for 1 h. A straight-catheter urine sample was obtained prior to and following the mindfulness-based stress reduction series. DNA from urine samples underwent bacterial 16S ribosomal gene sequencing at Johns Hopkins University Laboratories followed by taxonomic abundance and diversity analysis by Resphera Biosciences Laboratory. Participants completed validated symptom questionnaires pre- and post-intervention.
A total of 12 participants completed the 8-week course and were included in the analysis. The average age was 59 and the majority identified as white. Patient symptoms, measured by the Urogenital Distress Inventory Short Form and Interstitial Cystitis Symptom and Pain Indices, improved significantly (all p < 0.05). Overall composition of the urinary microbiome changed significantly (p < 0.01) and demonstrated an increase in diversity following the intervention.
Mindfulness-based stress reduction therapy improves patient symptoms and was associated with significant changes in the urinary microbiome in patients with interstitial cystitis/bladder pain syndrome.
本研究旨在探讨基于正念减压疗法对间质性膀胱炎/膀胱疼痛综合征患者尿液微生物群的影响。
在这项经机构审查委员会批准的前瞻性队列研究中，招募了间质性膀胱炎/膀胱疼痛综合征患者参加为期8周的包含瑜伽和冥想的正念减压课程。符合条件的参与者为年龄在18岁及以上、患有间质性膀胱炎/膀胱疼痛综合征且说英语的女性。所有参与者在入组后2个月内尿液分析结果均为阴性，且在招募时正在接受一线或二线治疗。基于正念减压的课程每周进行1小时。在正念减压课程系列前后分别采集一次导尿尿液样本。尿液样本的DNA在约翰·霍普金斯大学实验室进行细菌16S核糖体基因测序，随后由Resphera生物科学实验室进行分类丰度和多样性分析。参与者在干预前后完成经过验证的症状问卷。
共有12名参与者完成了为期8周的课程并纳入分析。平均年龄为59岁，大多数为白人。通过泌尿生殖系统困扰量表简表以及间质性膀胱炎症状和疼痛指数测量的患者症状有显著改善（所有p<0.05）。尿液微生物群的总体组成发生了显著变化（p<0.01），并且在干预后多样性增加。
基于正念减压疗法可改善间质性膀胱炎/膀胱疼痛综合征患者的症状，并与尿液微生物群的显著变化相关。
