Rojas L, Zuazaga C
Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan 00901.
Neurosci Lett. 1988 May 16;88(1):39-44. doi: 10.1016/0304-3940(88)90312-6.
The type of glass used to fabricate patch pipettes influences the kinetic properties of single-channel currents activated by acetylcholine in Xenopus myocytes. In patches formed with hard glass pipettes, the opening rate decreased in a time-dependent manner. Such decrease was most pronounced for the low-conductance channel. In addition, longer-duration events of this form of channel dropped out faster than shorter-duration events and, as a consequence, the apparent channel open time became shorter during the recording period. These effects were not observed in patches formed with soft glass pipettes.
用于制作膜片吸管的玻璃类型会影响非洲爪蟾肌细胞中由乙酰胆碱激活的单通道电流的动力学特性。在用硬质玻璃吸管形成的膜片中，开放速率呈时间依赖性下降。这种下降在低电导通道中最为明显。此外，这种通道形式的持续时间较长的事件比持续时间较短的事件更快消失，因此，在记录期间表观通道开放时间变短。在用软质玻璃吸管形成的膜片中未观察到这些效应。
