Saito Y, Kawashima S
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Neurosci Lett. 1988 Jun 17;89(1):102-7. doi: 10.1016/0304-3940(88)90488-0.
Specific protease-protease inhibitor interactions have been suggested to play a role in the regulation of neurite outgrowth. We have examined the influence of exogenously added specific protease inhibitors on neurite outgrowth in PC12h cell in serum-free defined medium. Of 14 protease inhibitor species examined, only a leupeptin analogue, Ac-Leu-Leu-Nle-al was stimulatory for neurite outgrowth in the presence of nerve growth factor. Leupeptin and soybean trypsin inhibitor which had been reported to induce neurite outgrowth from sensory ganglia and neuroblastoma cells had no effect. These results suggest that an endogenous protease of a new type is involved in restricting neurite outgrowth in PC12h cell.
特定的蛋白酶-蛋白酶抑制剂相互作用被认为在神经突生长的调节中发挥作用。我们已经研究了在无血清限定培养基中，外源性添加特定蛋白酶抑制剂对PC12h细胞神经突生长的影响。在所检测的14种蛋白酶抑制剂中，只有一种亮抑酶肽类似物Ac-Leu-Leu-Nle-al在神经生长因子存在的情况下对神经突生长具有刺激作用。据报道可诱导感觉神经节和神经母细胞瘤细胞产生神经突生长的亮抑酶肽和大豆胰蛋白酶抑制剂没有效果。这些结果表明，一种新型的内源性蛋白酶参与限制PC12h细胞的神经突生长。
