Onodera H, Tobita M, Kogure K
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Neurosci Lett. 1988 Jun 17;89(1):90-6. doi: 10.1016/0304-3940(88)90486-7.
CA1 pyramidal neurons are selectively vulnerable to ischemia. 2-[14C]Deoxyglucose (2-DG) autoradiography was employed to measure local glucose utilization in the rat hippocampus after transient ischemia, under the condition of unilateral prevention of ischemic CA1 pyramidal cell necrosis by unilateral lesioning of Schaffer collaterals (left side). Columnar 2-DG uptake patterns in the CA1 region with intact Schaffer collaterals (right side) was observed 19-22 h after recirculation, though no laterality was noted 3 and 8 h after recirculation. In the right subiculum, higher 2-DG uptake was observed 19-22 h after recirculation in 5 out of 8 animals. These results suggest that Schaffer collaterals play a role in modulating glucose metabolism in the CA1 and subiculum during recirculation.
CA1锥体神经元对缺血具有选择性易损性。采用2-[¹⁴C]脱氧葡萄糖（2-DG）放射自显影术，在通过单侧损毁Schaffer侧支（左侧）单侧预防缺血性CA1锥体细胞坏死的条件下，测量短暂缺血后大鼠海马体中的局部葡萄糖利用情况。再灌注19 - 22小时后，观察到Schaffer侧支完整的CA1区域（右侧）呈现柱状2-DG摄取模式，不过再灌注3小时和8小时后未发现左右侧差异。在右侧海马下脚，8只动物中有5只在再灌注19 - 22小时后观察到较高的2-DG摄取。这些结果表明，Schaffer侧支在再灌注期间对CA1和海马下脚的葡萄糖代谢调节中发挥作用。
