Departamento de Farmacobiología, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México City, Mexico.
Laboratorio de Neurobiología Conductual, Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City, Mexico; Escuela Internacional de Medicina, Universidad Anáhuac, Cancún, Mexico.
Horm Behav. 2021 Jun;132:104992. doi: 10.1016/j.yhbeh.2021.104992. Epub 2021 May 12.
The aim of this study was to evaluate female rat sexual motivation in a model of diabetes mellitus type 1. Severe hyperglycemia was induced in ovariectomized Wistar rats by injecting streptozotocin [STZ, 100 mg/kg, i.p.]. Ten days later, females received estradiol benzoate (10 μg/rat, s.c.) plus progesterone (3 mg/rat, s.c.). A group of STZ-treated animals was administered with insulin (2-4 U) every 12 h for 10 days, which normalized glucose levels. In the partner preference (PP) and sexual incentive motivation (SIM) tests, control females spent more time close to a sexually experienced male (SE) than with a castrated male (CM). STZ-treated females stayed the same amount of time with both stimuli, that is, they lost their sexual preference. We also evaluated the sense of smell using two behavioral tests, one related to sexual odors (SO) and another one to food odors (FO). In the SO test, control females spent more time sniffing the sawdust coming from cages that contained SE males; hyperglycemic females remained the same amount of time sniffing the sawdust of both stimuli: SE and CM. In the FO test, no differences were found between control and STZ-treated groups. Insulin treatment reverted the changes observed in hyperglycemic females in the PP, SIM and SO tests. These data suggest that severe hyperglycemia decreases sexual motivation and that insulin recovers such diminution.
本研究旨在评估 1 型糖尿病模型中雌性大鼠的性动机。通过腹腔注射链脲佐菌素（STZ，100mg/kg）诱导去卵巢 Wistar 大鼠发生严重高血糖。10 天后，雌性大鼠接受苯甲酸雌二醇（10μg/只，皮下注射）和孕酮（3mg/只，皮下注射）。一组 STZ 处理的动物每天接受 2-4U 胰岛素治疗 10 天，以使其血糖水平正常化。在伴侣偏好（PP）和性激励动机（SIM）测试中，对照组雌性大鼠与有性经验的雄性（SE）接近的时间比与去势雄性（CM）接近的时间多。STZ 处理的雌性大鼠与两种刺激物保持相同的时间，即它们失去了性偏好。我们还使用两种行为测试评估了嗅觉，一种与性气味（SO）有关，另一种与食物气味（FO）有关。在 SO 测试中，对照组雌性大鼠花更多的时间嗅探来自包含 SE 雄性大鼠的笼子的木屑；高血糖雌性大鼠对两种刺激物（SE 和 CM）的木屑嗅探时间保持不变。在 FO 测试中，对照组和 STZ 处理组之间没有发现差异。胰岛素治疗使高血糖雌性大鼠在 PP、SIM 和 SO 测试中观察到的变化得到逆转。这些数据表明，严重的高血糖会降低性动机，而胰岛素可以恢复这种降低。
