Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden; Department of Biomedical and Clinical Sciences, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden.
National Forensic Centre, Swedish Police Authority, Linköping, Sweden.
Forensic Sci Int Genet. 2021 Jul;53:102525. doi: 10.1016/j.fsigen.2021.102525. Epub 2021 May 8.
On the morning of October 19, 2004, an eight-year-old boy and a 56-year-old woman were stabbed to death on an open street in the city of Linköping, Sweden. The perpetrator left his DNA at the crime scene, and after 15 years of various investigation efforts, including more than 9000 interrogations and mass DNA screening of more than 6000 men, there were still no clues about the identity of the unknown murderer. The successful application of investigative genetic genealogy (IGG) in the US raised the interest for this tool within the Swedish Police Authority. After legal consultations it was decided that IGG could be applied in this double murder case as a pilot case study. From extensive DNA analysis, including whole-genome sequencing and genotype imputation, DNA data sets were established and searched within both GEDmatch and FamilyTree DNA genealogy databases. A number of fairly distant relatives were found from which family trees were created. The genealogy work resulted in two candidates, two brothers, one of whom matched the crime scene samples by routine STR profiling. The suspect confessed the murders at the initial police hearing and was later convicted of the murders. In this paper we describe the successful application of an emerging technology. We disclose details of the DNA analyses which, due to the poor quality and low quantity of the DNA, required reiterative sequencing and genotype imputation efforts. The successful application of IGG in this double murder case exemplifies its applicability not only in the US but also in Europe. The pressure is now high on the involved authorities to establish IGG as a tool for cold case criminal investigations and for missing person identifications. There is, however, a continuous need to accommodate legal, social and ethical aspects as well.
2004 年 10 月 19 日上午，一名 8 岁男孩和一名 56 岁妇女在瑞典林雪平市的一条开放街道上被刺死。凶手在犯罪现场留下了他的 DNA，经过 15 年的各种调查努力，包括 9000 多次审讯和对 6000 多名男子的大规模 DNA 筛查，仍然没有关于身份不明凶手的线索。在美国，侦查遗传系谱学（IGG）的成功应用引起了瑞典警方对此工具的兴趣。经过法律咨询，决定在这起双重谋杀案中应用 IGG 作为试点案例研究。通过广泛的 DNA 分析，包括全基因组测序和基因型推断，在 GEDmatch 和 FamilyTree DNA 家谱数据库中建立并搜索了 DNA 数据集。从大量的 DNA 分析中发现了一些相当远的亲属，从中创建了家谱。基因alogy 工作产生了两个候选者，两个兄弟，其中一个通过常规 STR 分析与犯罪现场样本匹配。嫌疑人在初次警方听证会上承认了谋杀案，并随后被判犯有谋杀罪。本文描述了一项新兴技术的成功应用。我们披露了 DNA 分析的细节，由于 DNA 质量差和数量低，需要反复进行测序和基因型推断。IGG 在这起双重谋杀案中的成功应用证明了它不仅在美国，而且在欧洲也具有适用性。现在，有关当局面临着很大的压力，需要将 IGG 确立为冷案犯罪调查和失踪人员识别的工具。然而，需要不断适应法律、社会和伦理方面的问题。
