Bacteriology, Department of Infectious Diseases, University of Liège, Liège, Belgium.
Internal Medicine, Companion Animal Clinic, Faculty of Veterinary Medicine, FARAH (Fundamental and Applied Research for Animals and Health) centre, Campus du Sart Tilman, University of Liège, Liège, Belgium.
Res Vet Sci. 2021 Jul;137:170-173. doi: 10.1016/j.rvsc.2021.04.032. Epub 2021 May 4.
Lactococcus (L.) garvieae is a zoonotic fish pathogen that can also cause bacteraemia and endocarditis in humans and has been isolated from healthy or diseased domestic animals. Nevertheless L. garvieae is more an opportunistic, than a primary pathogen since most affected humans have predisposing conditions and comorbidities. L. garvieae is also present in other animal species, most frequently cattle, but also sheep, goats, water buffaloes, and pigs, and much more rarely dogs, cats, horses, camel, turtle, snake and crocodile. The purpose of this study was to genomically (i) confirm the identification by MALDI-TOF MS® of a L. garvieae from the nasal discharge of a dog with chronic respiratory disorders and (ii) compare this canine isolate with human and animal L. garvieae isolates. According to the BLAST analysis after Whole Genome Sequencing, this canine isolate was more than 99% identical to 3 L. garvieae and belonged to a new Multi-Locus Sequence Type (ST45). MLST and whole genomes-based phylogenetic analysis were performed on the canine isolate and the 40 genomes available in Genbank. The canine L. garvieae was most closely related to an Australian camel and an Indian fish L. garvieae and more distantly to human L. garvieae. Twenty-five of the 29 putative virulence-associated genes searched for were detected, but not the 16 capsule-encoding genes. The heterogeneity of the L. garvieae species is reflected by the diversity of the MLSTypes and virulotypes identified and by the phylogenetic analysis.
缓症链球菌（L.）garvieae 是一种人畜共患的鱼类病原体，也可引起人类菌血症和心内膜炎，并已从健康或患病的家畜中分离出来。然而，缓症链球菌更多的是一种机会性病原体，而不是原发性病原体，因为大多数受影响的人都有潜在的条件和合并症。缓症链球菌也存在于其他动物物种中，最常见的是牛，但也有绵羊、山羊、水牛和猪，以及更罕见的狗、猫、马、骆驼、龟、蛇和鳄鱼。本研究的目的是通过 MALDI-TOF MS®（基质辅助激光解吸电离飞行时间质谱）对一只患有慢性呼吸道疾病的狗的鼻腔分泌物中的 L. garvieae 进行基因组（i）确认鉴定，并将该犬分离株与人类和动物 L. garvieae 分离株进行比较。根据全基因组测序后的 BLAST 分析，该犬分离株与 3 株 L. garvieae 的相似度超过 99%，属于一种新的多位点序列型（ST45）。对犬分离株和在 Genbank 中可用的 40 个基因组进行了 MLST 和全基因组系统发育分析。犬缓症链球菌与澳大利亚骆驼和印度鱼类缓症链球菌最为密切相关，与人类缓症链球菌的关系较远。在所搜索的 29 个推定的毒力相关基因中，检测到了 25 个，但未检测到 16 个荚膜编码基因。缓症链球菌物种的异质性反映在鉴定的 MLSTypes 和毒力型的多样性以及系统发育分析中。
