CHUK 基因多态性与汉族人群缺血性脑卒中的相关性研究。
Association of CHUK gene polymorphism and ischemic stroke in the Han Chinese population.
机构信息
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, Guangdong, China; Guangzhou University of Chinese Medicine, 510405 Guangzhou, Guangdong, China; University at Buffalo, The State University of New York, 14228 Buffalo, NY, USA; Guangxi University of Chinese Medicine, 530299 Nanning, Guangxi, China.
Guangxi University of Chinese Medicine, 530299 Nanning, Guangxi, China; The First Affiliated Hospital of Guangxi University of Chinese Medicine, 530023 Nanning, Guangxi, China.
出版信息
J Clin Neurosci. 2021 Jun;88:271-276. doi: 10.1016/j.jocn.2021.04.009. Epub 2021 Apr 23.
BACKGROUND
Recently, the pivotal role of component of inhibitor of nuclear factor kappa B kinase complex (CHUK) in lipid levels and blood pressure has been reported, and hypertension and hyperlipidemia are common risk factors of ischemic stroke (IS). However, the association between CHUK and IS has not yet been explored. This study aims at evaluating the relationship of CHUK polymorphisms (rs3808916, rs2230804 and rs3808917) and IS risk as well as IS-related risk factors.
METHODS
CHUK mRNA expression was detected between 53 IS patients and 53 healthy controls using quantitative real-time polymerase chain reaction (qRT-PCR). A total of 816 IS patients and 816 age- and sex-matched healthy controls were genotyped using the Sequenom MassARRAY iPLEX platform.
RESULTS
CHUK mRNA was highly expressed in IS patients compared with healthy subjects (P＜0.001). No significant associations were observed between rs3808916, rs2230804, rs3808917 and IS susceptibility (P＞0.05). Moreover, haplotype analysis showed that no haplotype of CHUK polymorphisms was associated with IS (P > 0.05). However, rs2230804 was related to diastolic blood pressure (DBP) of IS patients (P = 0.035), while rs3808917 was associated with triglyceride (TG) levels (P = 0.046).
CONCLUSIONS
The CHUK expression is involved in the development of IS. CHUK variants rs2230804, and rs3808917 may affect blood pressure and lipid levels of IS patients. However, CHUK rs3808916, rs2230804 and rs3808917 polymorphisms are not associated with IS risk.
背景
最近，核因子 kappa B 激酶复合物组成部分（CHUK）在血脂水平和血压中的关键作用已被报道，高血压和高血脂是缺血性中风（IS）的常见危险因素。然而，CHUK 与 IS 之间的关联尚未被探索。本研究旨在评估 CHUK 多态性（rs3808916、rs2230804 和 rs3808917）与 IS 风险以及 IS 相关危险因素的关系。
方法
采用实时定量聚合酶链反应（qRT-PCR）检测 53 例 IS 患者和 53 例健康对照者的 CHUK mRNA 表达。采用 Sequenom MassARRAY iPLEX 平台对 816 例 IS 患者和 816 名年龄和性别匹配的健康对照者进行基因分型。
结果
与健康对照组相比，IS 患者的 CHUK mRNA 表达水平显著升高（P＜0.001）。rs3808916、rs2230804、rs3808917 与 IS 易感性无显著相关性（P＞0.05）。此外，CHUK 多态性的单体型分析显示，无 CHUK 多态性单体型与 IS 相关（P＞0.05）。然而，rs2230804 与 IS 患者的舒张压（DBP）相关（P=0.035），而 rs3808917 与甘油三酯（TG）水平相关（P=0.046）。
结论
CHUK 表达参与 IS 的发生。CHUK 变体 rs2230804 和 rs3808917 可能影响 IS 患者的血压和血脂水平。然而，CHUK rs3808916、rs2230804 和 rs3808917 多态性与 IS 风险无关。
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