Shanxi Normal University, Linfen, Shanxi, 041004, People's Republic of China; Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response (Shanxi Normal University) in Shanxi Province, Linfen, Shanxi, 041000, People's Republic of China.
Chemosphere. 2021 Oct;281:130809. doi: 10.1016/j.chemosphere.2021.130809. Epub 2021 May 11.
As the ozone hole in the North and South poles continues to increase, the entire ecosystem will face an environmental crisis caused by enhanced UV-B radiation. Considering the function of TiO and the application of nanomaterials in agriculture, the effect of TiO-NPs on UV-B stress tolerance in Arabidopsis was investigated. The phenotype of plants was determined, and the expression patterns of antioxidant systems and related genes were analyzed. Modification of the antioxidant system and changes in the flavonoid content of plants were observed by histochemical staining. The effects of TiO-NPs and UV-B on mitosis were observed at the cellular level, and the degree of DNA damage was analyzed by the detection of CPDs content. The effects of TiO-NPs and UV-B on SOD isozymes were detected by SOD isozyme Native-PAGE electrophoresis. A laser confocal microscope was used to explore the protective mechanism of TiO-NPs against UV-B radiation. Results showed that pretreatment of TiO-NPs significantly alleviated the stress of UV-B radiation on plants. TiO-NPs activated the antioxidant system of plants, improved the activity of antioxidant enzymes, and promoted the synthesis of flavonoids. Moreover, TiO-NPs could effectively shield UV-B radiation to prevent the depolymerization of microtubules in plant cells. 10 mg/L of TiO-NPs is a safe and effective application dose, which has no biological toxicity to plants. Our research results reported for the first time that pretreatment of TiO-NPs could effectively alleviate UV-B stress to plants, providing new ideas for the application of nanomaterials in agriculture.
随着南北极臭氧空洞不断扩大，整个生态系统将面临因增强的 UV-B 辐射而引发的环境危机。考虑到 TiO 的功能以及纳米材料在农业中的应用，研究了 TiO-NPs 对拟南芥中 UV-B 胁迫耐受性的影响。测定了植物的表型，并分析了抗氧化系统和相关基因的表达模式。通过组织化学染色观察了植物抗氧化系统的修饰和类黄酮含量的变化。在细胞水平上观察了 TiO-NPs 和 UV-B 对有丝分裂的影响，并通过 CPDs 含量的检测分析了 DNA 损伤程度。通过 SOD 同工酶 Native-PAGE 电泳检测了 TiO-NPs 和 UV-B 对 SOD 同工酶的影响。利用激光共聚焦显微镜探讨了 TiO-NPs 对 UV-B 辐射的保护机制。结果表明，TiO-NPs 的预处理显著减轻了 UV-B 辐射对植物的胁迫。TiO-NPs 激活了植物的抗氧化系统，提高了抗氧化酶的活性，促进了类黄酮的合成。此外，TiO-NPs 可以有效地屏蔽 UV-B 辐射，防止植物细胞中微管的解聚。10mg/L 的 TiO-NPs 是一种安全有效的应用剂量，对植物没有生物毒性。我们的研究结果首次报道了 TiO-NPs 的预处理可以有效缓解植物的 UV-B 胁迫，为纳米材料在农业中的应用提供了新的思路。
