Zhang Qing, Tian Ping, Xu Hui
Department of Gynaecology, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan City, Hubei Province, 430014, China.
Department of Obstetrics, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan City, Hubei Province, 430014, China.
Reprod Biol. 2021 Sep;21(3):100510. doi: 10.1016/j.repbio.2021.100510. Epub 2021 May 13.
Recurrent miscarriage (RM) occurs in approximately 1% of all couples trying to conceive. Most of the research about recurrent miscarriage mainly focuses on immunology. However, the roles of microRNAs plays (miRNAs) in RM remain elusive. Here, the function of miR-155-5p in regulating survival of human decidua stromal cells through NF-κB signaling was explored in RM. The quantitative real-time polymerase chain reaction (qRT-PCR) results showed that miR-155-5p was downregulated in both decidua tissues and serum from RM patients. While, the ELISA assay revealed that the overexpression of miR-155-5p reduced the inflammatory cytokines secretion including IL-6, IFN-γ, TNF-α and IL-10 in decidua stromal cells. The results of cell counting Kit8 (CCK-8) and immunofluorescence experiments suggested that transfection of miR-155-5p into decidua stromal cells can promote the growth and proliferation of cells. In addition, overexpression of miR-155-5p can also inhibit the apoptosis of decidua stromal cells. The western blot assay results demonstrated that the miR-155-5p exerted effect mainly through activating NF-κB signaling pathway in RM. In conclusion, the miRNA-155-5p can not only promote the growth and proliferation but also inhibit the apoptosis of decidua stromal cells depending on inhibiting NF-κB signaling pathway in recurrent miscarriage.
复发性流产（RM）发生在约1%试图受孕的夫妇中。关于复发性流产的大多数研究主要集中在免疫学方面。然而，微小RNA（miRNA）在复发性流产中的作用仍不清楚。在此，研究了miR-155-5p在复发性流产中通过NF-κB信号通路调节人蜕膜基质细胞存活的功能。定量实时聚合酶链反应（qRT-PCR）结果显示，复发性流产患者的蜕膜组织和血清中miR-155-5p均下调。同时，酶联免疫吸附测定（ELISA）显示，miR-155-5p的过表达减少了蜕膜基质细胞中包括白细胞介素-6（IL-6）、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）在内的炎性细胞因子分泌。细胞计数试剂盒8（CCK-8）和免疫荧光实验结果表明，将miR-155-5p转染到蜕膜基质细胞中可促进细胞生长和增殖。此外，miR-155-5p的过表达还可抑制蜕膜基质细胞的凋亡。蛋白质印迹分析结果表明，miR-155-5p在复发性流产中主要通过激活NF-κB信号通路发挥作用。总之，在复发性流产中，miRNA-155-5p不仅可以促进蜕膜基质细胞的生长和增殖，还可以通过抑制NF-κB信号通路抑制其凋亡。
