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长链非编码RNA ZNF674-AS1通过与醛缩酶A相互作用调控颗粒细胞糖酵解和增殖。

LncRNA ZNF674-AS1 regulates granulosa cell glycolysis and proliferation by interacting with ALDOA.

作者信息

Li Duan, Wang Xiaoyan, Li Guangyu, Dang Yujie, Zhao Shidou, Qin Yingying

机构信息

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, China.

Key laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, 250012, Jinan, Shandong, China.

出版信息

Cell Death Discov. 2021 May 16;7(1):107. doi: 10.1038/s41420-021-00493-1.

DOI:10.1038/s41420-021-00493-1
PMID:33993190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8124069/
Abstract

Granulosa cell (GC) is a critical somatic component of ovarian follicles to support oocyte development, while the regulatory role of long noncoding RNA (lncRNA) in GCs is largely unknown. Here, we identified a down-regulated lncRNA ZNF674-AS1 in GCs from patients with biochemical premature ovarian insufficiency (bPOI), and its expression correlates with serum levels of clinical ovarian reserve indicators. Functional experiments showed that ZNF674-AS1 is induced by energy stress, and regulates the proliferation and glycolysis of GCs, which possibly leads to follicular dysfunction. Mechanistically, low-expressed ZNF674-AS1 reduced the enzymatic activity of aldolase A (ALDOA), concomitant with promoting the association between ALDOA and v-ATPase to activate the lysosome localized AMP-activated protein kinase (AMPK). These findings identified a new lncRNA-ALDOA complex through which ZNF674-AS1 exerts its functions, expanding the understanding of epigenetic regulation of GCs function and POI pathogenesis.

摘要

颗粒细胞（GC）是卵巢卵泡中支持卵母细胞发育的关键体细胞成分，而长链非编码RNA（lncRNA）在颗粒细胞中的调控作用在很大程度上尚不清楚。在此，我们在生化性卵巢早衰（bPOI）患者的颗粒细胞中鉴定出一种下调的lncRNA ZNF674-AS1，其表达与临床卵巢储备指标的血清水平相关。功能实验表明，ZNF674-AS1受能量应激诱导，并调节颗粒细胞的增殖和糖酵解，这可能导致卵泡功能障碍。机制上，低表达的ZNF674-AS1降低了醛缩酶A（ALDOA）的酶活性，同时促进ALDOA与v-ATP酶之间的结合，以激活定位于溶酶体的AMP激活蛋白激酶（AMPK）。这些发现确定了一种新的lncRNA-ALDOA复合物，ZNF674-AS1通过该复合物发挥其功能，扩展了对颗粒细胞功能和POI发病机制的表观遗传调控的理解。

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3
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Reprod Sci. 2025 Apr 2. doi: 10.1007/s43032-025-01854-2.
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