Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
J Assist Reprod Genet. 2021 Sep;38(9):2349-2361. doi: 10.1007/s10815-021-02229-7. Epub 2021 May 15.
Implantation is essential for a successful pregnancy. Despite the increasing number of studies, implantation is still an unknown process. This study aimed to determine whether sirtuin-1 has a role in embryo implantation in oxidative stress-induced mice.
Pregnant mice were separated into 5 groups: control, vehicle, paraquat, SRT1720, and SRT1720+Paraquat. Paraquat is a herbicide and is used to induce oxidative stress. SRT1720 is a specific sirtuin-1 activator. Implantation and inter-implantation sites were removed in the morning of the 5th day of pregnancy after Chicago blue injection was performed. Sirtuin-1 and Forkhead box O1 (FoxO1) were detected by immunohistochemistry and Western blot while acetylated lysine was evaluated by Western blot analysis. Reactive oxygen and nitrogen species (ROS/RNS) and superoxide dismutase (SOD) activity were determined by fluorometric and spectrometric methods, respectively.
Although there was no embryo implantation in paraquat-treated mice, 5 out of 9 SRT1720+Paraquat-treated mice had implantation sites which were significantly higher compared to the paraquat-treated group. Sirtuin-1 and FoxO1 expressions were increased at implantation sites of SRT1720-treated mice. ROS/RNS levels were decreased, while deacetylated FoxO1 levels and SOD activity were increased in SRT1720-treated mice.
Our findings suggest that sirtuin-1 may play a role in embryo implantation against oxidative stress through FoxO1-SOD signaling.
着床对于成功妊娠至关重要。尽管研究越来越多，但着床仍然是一个未知的过程。本研究旨在确定 Sirtuin-1 在氧化应激诱导的小鼠胚胎着床中是否发挥作用。
将怀孕的小鼠分为 5 组：对照组、载体组、百草枯组、SRT1720 组和 SRT1720+百草枯组。百草枯是一种除草剂，用于诱导氧化应激。SRT1720 是一种特异性 Sirtuin-1 激活剂。在怀孕第 5 天早晨，通过芝加哥蓝注射后，取出着床和着床之间的部位。通过免疫组织化学和 Western blot 检测 Sirtuin-1 和 Forkhead box O1 (FoxO1)，通过 Western blot 分析评估乙酰化赖氨酸。通过荧光法和分光光度法分别测定活性氧和氮物种（ROS/RNS）和超氧化物歧化酶（SOD）活性。
尽管百草枯处理的小鼠没有胚胎着床，但 9 只 SRT1720+百草枯处理的小鼠中有 5 只出现了着床部位，明显高于百草枯处理组。Sirtuin-1 和 FoxO1 的表达在 SRT1720 处理的小鼠着床部位增加。SRT1720 处理的小鼠 ROS/RNS 水平降低，而去乙酰化 FoxO1 水平和 SOD 活性增加。
我们的研究结果表明，Sirtuin-1 可能通过 FoxO1-SOD 信号通路在对抗氧化应激的胚胎着床中发挥作用。
