Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.
Homi Bhabha National Institute, Mumbai, India.
FEBS Lett. 2021 Jul;595(13):1844-1860. doi: 10.1002/1873-3468.14108. Epub 2021 May 30.
Microplasts are large extracellular vesicles originating from migratory, invasive, and metastatic cancer cells. Here, to gain insight into the role of microplasts in cancer progression, we performed a proteomic and transcriptomic characterization of microplasts isolated from MCF-7 breast cancer cells treated with macrophage-conditioned medium. These cells were found to be viable, highly migratory, and metabolically active, indicating that microplasts derived from these cells are not apoptotic bodies. Transcriptomic/proteomic analyses identified 10273 mRNAs and 821 proteins in microplasts. Interestingly, 377 microplast mRNAs coded for corresponding microplast proteins. Microplast mRNAs and proteins were mainly associated with binding and catalytic activities. Microplasts showed enrichment of mRNAs involved in transcription regulation and proteins involved in processes such as cell-cell adhesion and translation. Pathway analysis showed enrichment of ribosomes and carbon metabolism. These results suggest a close resemblance between microplasts and parent cells, with mRNA and protein cargo relevant in intercellular signaling.
微塑料是源自迁移、侵袭和转移性癌细胞的大型细胞外囊泡。在这里，为了深入了解微塑料在癌症进展中的作用，我们对用巨噬细胞条件培养基处理的 MCF-7 乳腺癌细胞中分离的微塑料进行了蛋白质组学和转录组学表征。这些细胞被发现是有活力的、高度迁移的和代谢活跃的，这表明这些细胞衍生的微塑料不是凋亡小体。转录组/蛋白质组学分析鉴定了微塑料中的 10273 个 mRNAs 和 821 个蛋白质。有趣的是，377 个微塑料 mRNAs 编码相应的微塑料蛋白质。微塑料的 mRNAs 和蛋白质主要与结合和催化活性有关。微塑料表现出与转录调控相关的 mRNAs 和与细胞-细胞粘附和翻译等过程相关的蛋白质的富集。通路分析显示核糖体和碳代谢的富集。这些结果表明微塑料与母细胞非常相似，mRNA 和蛋白质货物与细胞间信号传递有关。
