Nikitina N V, Leonov I K, Yavdoshak L I
FSBRI «All-Russian Research Veterinary Institute of Poultry Science» - Branch of the FSBRI FSC «All-Russian Research and Technological Poultry Institute» of Russian Academy of Sciences.
Vopr Virusol. 2021 May 15;66(2):162-166. doi: 10.36233/0507-4088-43.
Duck viral hepatitis type I (DVH-I) is a poorly studied contagious disease caused by RNA-containing duck (Anatinae) hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A). This infection is widespread in many countries, including Russia, and causes significant damage to industrial duck breeding. The study of interferonogenic activity of its etiologic agent strains is of great importance in solving the problem of developing effective means to control the disease.
Strain BH-3 of duck hepatitis virus type I isolated from the liver of sick ducklings was used in the study. The strain was adapted to developing 10-12 day old duck embryos, to the cell culture of chicken and duck fibroblasts and deposited in the State Collection of Viruses of the D.I. Ivanovsky Institute of Virology of FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of Russia. Experiments were performed using the standard tissue culture method.
Data on the ability of the viral strain BH-3 to induce interferon (IFN) and its sensitivity to the action of exogenous interferon in the culture of duck fibroblasts are presented. It has been shown that the interferonogenic activity of this strain of the hepatitis virus is in direct proportion to the multiplicity of infection. The maximum induction of IFN (1 : 256 CEPD50) was observed at a dose of 1.0 TCD50/cell in 72-96 hrs after inoculation of the cell culture. Exogenous IFN at a dose of 1 : 128 completely suppressed the cytopathic effect and death of duck embryos infected with hepatitis virus at a dose of 100 TCD50/cell.
The data obtained allow us to state that the vaccine strain BH-3 of duck hepatitis virus type I has a pronounced interferonogenic activity and sensitivity to the action of exogenous IFN. This may have implications for the development of effective therapeutic agents against DVH-I.
I型鸭病毒性肝炎（DVH-I）是一种研究较少的传染病，由含RNA的I型鸭（鸭科）肝炎病毒引起（小RNA病毒科：禽肝炎病毒属：禽肝炎病毒A）。这种感染在包括俄罗斯在内的许多国家广泛存在，对工业鸭养殖造成重大损害。研究其病原体菌株的干扰素诱导活性对于开发控制该疾病的有效手段至关重要。
本研究使用从患病雏鸭肝脏分离的I型鸭肝炎病毒BH-3株。该菌株适应于10 - 12日龄鸭胚、鸡和鸭成纤维细胞培养，并保藏于俄罗斯联邦卫生部FSBI“以荣誉院士N.F.加马列亚命名的国家流行病学和微生物学研究中心”的D.I.伊万诺夫斯基病毒研究所国家病毒保藏库。实验采用标准组织培养方法进行。
给出了病毒株BH-3在鸭成纤维细胞培养中诱导干扰素（IFN）的能力及其对外源干扰素作用敏感性的数据。已表明该肝炎病毒株的干扰素诱导活性与感染复数成正比。在细胞培养接种后72 - 96小时，当剂量为1.0 TCD50/细胞时，观察到IFN的最大诱导量（1 : 256 CEPD50）。剂量为1 : 128的外源IFN完全抑制了以100 TCD50/细胞剂量感染肝炎病毒的鸭胚的细胞病变效应和死亡。
所获得的数据使我们能够指出，I型鸭肝炎病毒疫苗株BH-3具有明显的干扰素诱导活性以及对外源IFN作用的敏感性。这可能对开发抗DVH-I的有效治疗剂具有重要意义。
