University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary Clinical Sciences, DK Taastrup.
ScanVet Animal Health A/S, DK Fredensborg.
J Equine Vet Sci. 2021 Jun;101:103435. doi: 10.1016/j.jevs.2021.103435. Epub 2021 Mar 8.
Alternative treatment options to glucocorticoids for equine asthma is desirable due to withdrawal time. The objective was to evaluate if serum and Respiratory Gene Technology (RGT), a commercial kit to produce autologous conditioned serum, was effective in reducing bronchoalveolar lavage (BAL) neutrophils and mast cells in racehorses with cytological evidence of mild equine asthma . Thirty-six Standardbred trotters in active training were enrolled in this randomized clinical trial; a healthy control group (n=11), a RGT group (n=12) and a serum group (n=13). Endoscopy including tracheal wash (TW) and BAL was performed before (T0), after a 6-week treatment period including 12 intramuscular injections of RGT or serum (T6) and as a follow-up 10 weeks after treatment (T16). A significant decrease in BAL neutrophils for the RGT group was found between T0 and T6 (P = .002, d=-1.51, CI: -2.43;-0.59) and for the serum group between T0-T6 (P = .002, d=-1.36, CI: -2.26;-0.46). Further, a significant decrease in BAL mast cells between T0-T6 for the both the RGT group (P = .019, d=-1.23, CI: -1.22;-0.34) and the serum group (P= .004, d=-0.81, CI: -1.65;0.04), and further between T0-T16 (RGT P= .011, d=-1.55, CI: -2.62;-0.48; serum P= .044, d=-0.65, CI: -1.68;-0.37). No significant difference in TW cytology was found for any of the time-points. Pro- and anti-inflammatory cytokines were regulated according to treatment. The control group showed no cytological differences between any time-point. Study results showed that intramuscular treatment with both RGT and serum was effective associated with reduction of BAL neutrophils and mast cells in horses with cytological evidence of mild equine asthma. Further large-scale studies are necessary.
由于停药时间的原因，寻找糖皮质激素的替代治疗方案是很有必要的。本研究的目的是评估血清和呼吸基因技术（RGT），一种用于产生自体条件血清的商业试剂盒，是否能有效降低有细胞学证据的轻度马气喘马匹的支气管肺泡灌洗液（BAL）中性粒细胞和肥大细胞。本随机临床试验共纳入 36 匹处于活跃训练中的标准纯血马；健康对照组（n=11）、RGT 组（n=12）和血清组（n=13）。在治疗前（T0）、治疗 6 周（包括 12 次肌肉注射 RGT 或血清）后（T6）以及治疗后 10 周（T16）进行内窥镜检查，包括气管冲洗（TW）和 BAL。RGT 组在 T0 和 T6 之间 BAL 中性粒细胞显著下降（P=0.002，d=-1.51，CI：-2.43；-0.59），血清组在 T0-T6 之间 BAL 中性粒细胞显著下降（P=0.002，d=-1.36，CI：-2.26；-0.46）。此外，RGT 组和血清组在 T0-T6 之间 BAL 肥大细胞均显著下降（RGT 组 P=0.019，d=-1.23，CI：-1.22；-0.34；血清组 P=0.004，d=-0.81，CI：-1.65；0.04），并且在 T0-T16 之间进一步下降（RGT 组 P=0.011，d=-1.55，CI：-2.62；-0.48；血清组 P=0.044，d=-0.65，CI：-1.68；-0.37）。在任何时间点，TW 细胞学均无显著差异。治疗后，促炎和抗炎细胞因子均得到调节。对照组在任何时间点均无细胞学差异。研究结果表明，肌肉内注射 RGT 和血清均可有效降低有细胞学证据的轻度马气喘马匹的 BAL 中性粒细胞和肥大细胞。还需要进一步的大规模研究。
